Wyniki wyszukiwania

1

Analysis of in vivo- and in vitro-derived pig expanded blastocysts based on DNA fragmentation

100%

Bryla M., Trzcinska M., Wieczorek J.

Animal Science Papers and Reports

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2009

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tom 27

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nr 1

59-68

The aim of the study was to investigate the competence of in vivo- and in vitro-derived pig expanded blastocysts by analysing DNA fragmentation using TUNEL. A total of 533 porcine expanded blastocysts were examined, and results were evaluated using Fisher’s test. Significant differences in the incidence of fragmented nuclei (detected by the TUNEL reaction) and all nuclei (detected by DAPI) were identified between in vivo- and in vitro-derived embryos at the expanded blastocyst stage. The total numbers of nuclei observed in in vivo-derived embryos were significantly different from those in in vitro-cultured embryos (89.1±13.4 and 47.7±25.1, respectively). TUNEL index in In vitro-cultured embryos (28.3%) was significantly higher (P<0.01) than in in vivo-derived blastocysts (4%). These findings indicate that in vivo- and in vitro-derived expanded blastocysts consisting of a small number of cells are characterized by a high incidence of DNA fragmentation. The total number of nuclei in in vivo- and in vitro-derived blastocysts correlated negatively with the number of TUNEL-positive nuclei (r = -0.51; P<0.0001) and the TUNEL index (r = -0.69; P<0.0001), whereas the number of TUNEL-positive nuclei was positively correlated with the TUNEL index (r = 0.95;P<0.0001). Moreover, significant differences were observed between embryos collected from individual experiments.

2

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

100%

Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.

Folia Morphologica

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2005

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tom 64

|

nr 4

248-252

Intracerebral haematoma was produced in 25 adult rats by infusion of 100 µl of autologous blood into the striatum. The animals’ brains were removed at 1, 3, 7, 14 and 21 days after production of the haematoma. The TUNEL method was used to detect DNA fragmentation and TUNEL-positive cells were qualified. TUNEL-positive cells were already found on the first day of observation and were present for three weeks after haematoma production. These results provide evidence that programmed cell death is associated with intracerebral haemorrhage.

3

The induction of nuclear DNA breaks in human skin cells by ultraviolet a [UVA] light

100%

Brozyna A., Kwiatkowska K., Chwirot B. W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Apoptosis and cytotoxicity caused by ethoxyquin and two of its salts

100%

Blaszczyk A., Skolimowski J.

Cellular and Molecular Biology Letters

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2005

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tom 10

|

nr 1

5

A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

84%

Dereli M., Krazinski B. E., Ayvaz S., Aksu B., Kanter M., Uzun H., Gelisgen R., Umit C. H., Inan M., Basaran U. N., Pul M.

Folia Histochemica et Cytobiologica

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2014

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tom 52

|

nr 1

6

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Effect of Solcoseryl on the apoptosis in the lungs of experimentally irradiated rats

84%

Staroslawska E., Donica H., Kozaczynski W., Wojcierowski J., Jarmolinska K., Wolanska E., Bozyk A.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

Modifying effect of Solcoseryl upon the late postirradiation damage of the lungs expressed as the number of apoptotic cells per 1mm² of the pulmonary tissue was investigated in rats. The number of labelled apoptotic cells in situ was determined by TUNEL method under light microscope connected to computer imaging (Lucia software). The results of the experiment demonstrated statistically significant reduction in the number of apoptotic cells in the pulmonary tissue in the group of rats irradiated with simultaneous administration of Solcoseryl in comparison to the group of animals irradiated without Solcoseryl treatment. The results indicate that Solcoseryl exhibits radioprotective activity upon the respiratory epithelium due to the reduction of the apoptotic cell number.

7

Fluoro-Jade and TUNEL staining as useful tools to identify ischemic brain damage following moderate extradural compression of sensorimotor cortex

84%

Kundrotiene J., Wagner A., Liljequist S.

Acta Neurobiologiae Experimentalis

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2004

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tom 64

|

nr 2

8

Application of biotinylated tyramine to amplify reactions with horseradish peroxidase labelling

84%

Surowiak P., Zabel M., Kasprzak A., Seidel J., Surdyk-Zasada J., Lesisz I.

Folia Histochemica et Cytobiologica

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1999

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tom 37

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nr 2

9

Adult respiratory distress syndrome and alveolar epithelium apoptosis: an histopathological and immunohistochemical study

67%

Toth S., Pingorova S., Jonecova Z., Morochovic R., Pomfy M., Vesela J.

Folia Histochemica et Cytobiologica

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2009

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tom 47

|

nr 3

10

The quality of porcine oocytes is affected by sexual maturity of the donor gilt

67%

Pawlak P., Renska N., Pers-Kamczyc E., Warzych E., Lechniak D.

Reproductive Biology

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2011

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tom 11

|

nr 1

11

Apoptosis in preimplantation bovine embryos and methods used for its detection

67%

Opiela J.

Annals of Animal Science

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2009

|

tom 09

|

nr 1

3-16

12

The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia

67%

Nowicki R., Saczko J., Kulbacka J., Choromanska A., Dumanska M., Krajewska B., Daczewska M., Dumanski A., Kustrzycki W.

Folia Histochemica et Cytobiologica

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2010

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tom 48

|

nr 2

202-207

13

Expression of the membrane complement regulatory proteins [CD55 and CD59] in human thymus

67%

Sladowski D., Wasiutynski A., Wilczynski G., Grabska-Liberek I., Coecke S., Kinsner A., Kochanowska I.

Folia Histochemica et Cytobiologica

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2006

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tom 44

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nr 4

14

Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina

67%

Dezawa M., Mo X., Oshitari T., Takano M., Meyer-Rochow V. B., Sawada H., Eguchi E.

Acta Neurobiologiae Experimentalis

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2001

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tom 61

|

nr 2

15

Postnatal development of the rat striatum - a study using in situ DNA end labeling technique

67%

Maciejewska B., Lipowska M., Kowianski P., Domaradzka-Pytel B., Morys J.

Acta Neurobiologiae Experimentalis

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1998

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tom 58

|

nr 1

Ograniczanie wyników

Analysis of in vivo- and in vitro-derived pig expanded blastocysts based on DNA fragmentation

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

The induction of nuclear DNA breaks in human skin cells by ultraviolet a [UVA] light

Apoptosis and cytotoxicity caused by ethoxyquin and two of its salts

A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Effect of Solcoseryl on the apoptosis in the lungs of experimentally irradiated rats

Fluoro-Jade and TUNEL staining as useful tools to identify ischemic brain damage following moderate extradural compression of sensorimotor cortex

Application of biotinylated tyramine to amplify reactions with horseradish peroxidase labelling

Adult respiratory distress syndrome and alveolar epithelium apoptosis: an histopathological and immunohistochemical study

The quality of porcine oocytes is affected by sexual maturity of the donor gilt

Apoptosis in preimplantation bovine embryos and methods used for its detection

The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia

Expression of the membrane complement regulatory proteins [CD55 and CD59] in human thymus

Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina

Postnatal development of the rat striatum - a study using in situ DNA end labeling technique