Wyniki wyszukiwania

1

The cellular architecture of the pterygopalatine ganglion of Syrian hamster [Mesocricetus auratus W. 1839]

100%

Kuder T., Szczurkowski A.

Zoologica Poloniae

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1996

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tom 41

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nr 1-4

2

Cytoarchitectonics of the ganglia functionally connected with the chorda tympani of the Syrian hamster

86%

Kuder T., Szczurkowski A.

Folia Morphologica

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1997

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tom 56

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nr 3

3

Effect of prolonged and shortened photoperiods on motor activity in Syrian hamsters (Mesocricetus auratus Waterhouse) exposed to additional negative ionization of air

72%

Lenkiewicz Z., Dabrowska B., Schiffer Z.

Acta Biologica Cracoviensia. Series Zoologia

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1989

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tom 31

4

Dynamics of estrogen-induced oxidative stress

72%

Kobiela J., Stefaniak T., Krajewski J., Kalinska-Blach B., Zurawa-Janicka D., Lachinski A., Gackowski D., Olinski R., Nowak J., Knap N., Lipinska B., Sledzinski Z., Wozniak M.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

The objective of this study was to assess the dynamics of oxidative damage to cellular macromolecules such as proteins, lipids and DNA under conditions of oxidative stress triggering early stages of estrogen-dependent carcinogenesis. A rodent model of carcinogenesis was used. Syrian hamsters were sacrificed after 1, 3, 5 h and one month from the initial implantation of estradiol. Matching control groups were used. Kidneys as target organs for estradiol-mediated oxidative stress were excised and homogenized for biochemical assays. Subcellular fractions were isolated. Carbonyl groups (as a marker of protein oxidation) and lipid hydroxyperoxides were assessed. DNA was isolated and 8-oxodGuo was assessed. Electron paramagnetic resonance spectroscopy was used to confirm the results for lipid peroxidation. Exposition to estradiol in the rodent model leads to damage of macromolecules of the cell, including proteins and DNA, but not lipids. Proteins appear to be the primary target of the damage but are closely followed by DNA. It has previously been speculated that protein peroxides can increase DNA modifications. This time sequence was observed in our study. Nevertheless, the direct relation between protein and DNA damage still remains unsolved.

5

Selectivity of oxidative stress targeting in estrogen-induced experimental nephrocarcinogenesis

72%

Kobiela J., Krajewski J., Kalinska-Blach B., Stefaniak T.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

Specificity of targeting of the oxidative stress towards lipid and protein fractions in a model of estrogen-induced Syrian hamster nephrocarcinogenesis was evaluated. The amount of proteins modified by oxidative stress was significantly elevated as early as one month after the initial implantation of estradiol to the experimental versus the control group, while the stress did not affect lipids. Subcellular localization of the oxidative stress target was determined by the analysis of protein oxidation in subcellular fractions of kidney cells. The endoplasmic reticulum membranes were the fraction most affected by the oxidative stress.

6

Co-infection of hamsters with toxin A or toxin B-deficient Clostridium difficile strains

72%

Szczesny A., Martirosian G., Cohen S., Silva J.,Jr.

Polish Journal of Microbiology

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2005

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tom 54

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nr 4

Male Syrian hamsters (Mesocricetus auratus) were used to study interactions between different toxin deficient strains of C. difficile. After sensitization with clindamycin, hamsters were intragastrically co-infected with the appropriate dilutions corresponding to 100, 1000 and 10,000 cells of four (toxin A or B-deficient) C. difficile strains (8864, P-829, W-38 and W-74). In addition, a group of hamsters was infected with C. difficile VPI 10463, a reference toxigenic strain. Colonization and mortality was observed within 48 hours in the group of hamsters infected with the reference toxigenic strain. No clinical disease was observed in the groups of hamsters co-infected with the toxin A or B-deficient strains. Re-infection of these hamsters (co-infected with toxin deficient isolates) with C. difficile VPI 10463 resulted in clinical disease and death suggesting that these strains do not confer protection against infection with a toxigenic strain. Macroscopic and microscopic observations of the cecum of re-infected hamsters demonstrated uniformly multiple large hemorrhagic areas without pseudomembranes. Hamsters infected with as few as 100-500 cells of the toxigenic strain - VPI 10463 alone demonstrated pseudomembranes and multiple hemorrhages. These results suggest that even though the toxin deficient strains did not prevent re-infection with a toxigenic strain of C. difficile, they may play a role in the histopathologic changes after re-infections in the hamster model. Further studies with a larger number of hamsters and C. difficile strains of various molecular profiles are required to better understand the interaction between these strains.

7

The malignant transformation of Syrian hamster embryo [SHE] cells in primary culture by malachite green: the transformation is associated with enhanced vimentin phosphorylation, PCNA expression and BrdU incorporation

58%

Mahudawala D. M., Redkar A. A., Rao K. V. K.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 1

Malachite green (MG) consisting of green crystals with a metallic lustre, is very soluble in water and is highly cytotoxic to mammalian cells and also acts as a liver tumor promoter. In view of its industrial importance and possible exposure to human beings, MG poses a potential environmental health hazard. The malignant transformation of Syrian hamster embryo (SHE) cells by MG has been reported earlier. In this study, an attempt has been made to study the levels of vimentin, vimentin phosphorylation and the expression of PCNA and BrdU incorporation in MG transformed cells compared to control cells. Immunohistochemical and immunoprecipitation studies showed enhanced levels of vimentin in transformed cells compared to normal cells. Metabolic labelling studies showed an overall increase in phosphorylation of total cellular proteins as well as hyperphosphorylation of vimentin in transformed cells. Transformed cells also showed an increased doubling time, PCNA expression and BrdU incorporation. This study indicates a close relationship between vimentin levels, hyperphosphorylation of vimentin and increased cell proliferation associated with the malignant transformation of SHE cells.

8

Changes in expression of serine proteases HtrA1 and HtrA2 during estrogen-induced oxidative stress and nephrocarcinogenesis in male Syrian hamster

58%

Zurawa-Janicka D., Kobiela J., Stefaniak T., Wozniak A., Narkiewicz J., Wozniak M., Limon J., Lipinska B.

Acta Biochimica Polonica

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2008

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tom 55

|

nr 1

Serine proteases HtrA1 and HtrA2 are involved in cellular stress response and development of several diseases, including cancer. Our aim was to examine the involvement of the HtrA proteins in acute oxidative stress response induced in hamster kidney by estrogen treatment, and in nephrocarcinogenesis caused by prolonged estrogenization of male Syrian hamster. We used semi-quantitative RT-PCR to estimate the HtrA1 and HtrA2 mRNA levels in kidney tissues, and Western blotting to monitor the amount of the HtrA proteins. Within the first five hours following estrogen administration both HtrA1 mRNA and the protein levels were increased significantly. No changes in the expression of HtrA2 were observed. This indicates that HtrA1 may be involved in the response against oxidative stress induced by estrogen treatment in hamster kidney. During prolonged estrogenization, a significant reduction of the HtrA1 mRNA and protein levels was observed after 6 months of estradiol treatment, while the expression of HtrA2 was significantly elevated starting from the third month. This suggests an involvement of the HtrA proteins in estrogen-induced nephrocarcinogenesis in hamster. Using fluorescence in situ hybridization we localized the HtrA1 gene at the qb3-4 region of Syrian hamster chromosome 2, the region known to undergo a nonrandom deletion upon prolonged estrogenization. It is possible that the reduced level of HtrA1 expression is due to this chromosomal aberration. A full-length cDNA sequence of the hamster HtrA1 gene was obtained. It codes for a 50 kDa protein which has 98 and 96% identity with mouse and human counterparts, respectively.

9

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Oxytocin and prolactin release after hypertonic saline administration in melatonin-treated male syrian hamsters

51%

Juszczak M., Steger R. W., Fadden C., Bartke A.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 2

10

Phenotypic transformation of hamster kidney cells by extracts of airborne pollutants from Upper Silesia

51%

Motykiewicz G., Szeliga J., Seemayer N. H.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1995

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tom 43

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nr 2

Ograniczanie wyników

The cellular architecture of the pterygopalatine ganglion of Syrian hamster [Mesocricetus auratus W. 1839]

Cytoarchitectonics of the ganglia functionally connected with the chorda tympani of the Syrian hamster

Effect of prolonged and shortened photoperiods on motor activity in Syrian hamsters (Mesocricetus auratus Waterhouse) exposed to additional negative ionization of air

Dynamics of estrogen-induced oxidative stress

Selectivity of oxidative stress targeting in estrogen-induced experimental nephrocarcinogenesis

Co-infection of hamsters with toxin A or toxin B-deficient Clostridium difficile strains

The malignant transformation of Syrian hamster embryo [SHE] cells in primary culture by malachite green: the transformation is associated with enhanced vimentin phosphorylation, PCNA expression and BrdU incorporation

Changes in expression of serine proteases HtrA1 and HtrA2 during estrogen-induced oxidative stress and nephrocarcinogenesis in male Syrian hamster

Oxytocin and prolactin release after hypertonic saline administration in melatonin-treated male syrian hamsters

Phenotypic transformation of hamster kidney cells by extracts of airborne pollutants from Upper Silesia