This investigation was conducted to evaluate the effects of four strains of plant growth promoting rhizobacteria (Agrobacterium rubi A-18, Bacillus subtilis OSU- 142, Burkholderia gladioli OSU-7 and Pseudomonas putida BA-8) on growth and leaf nutrient content of 'Starking Delicious', 'Granny Smith', 'Starkrimson Delicious', 'Starkspur Golden Delicious' and 'Golden Delicious' apple cultivars grafted on semi-dwarf rootstock MM-106. The applications of bacterial strains increased the leaf number and area as well as number of annual shoots and their diameter, although OSU-7 application suppressed annual shoot length. BA-8 application resulted in the highest annual shoot number (52.4) and OSU-142 in the largest leaf area (16.12 cm2). The applications of A-18 bacteria decreased the concentration of N, K and Cu and increased the concentration of P and Zn in the leaves. OSU-142 application resulted in the highest Mg (0.13%) and Fe (32.7 ppm) contents and OSU-7 in the highest Mn content (40.3 ppm). None of the applications affected the concentration of Na and Ca in the leaves.