Scutellaria barbata D.Don is an important medicinal plant used in traditional Chinese phytotherapy. It has been recently acclimated with success to Central European climatic conditions. Here, we present the results of chromatographic studies of herb collected from plants cultivated in Poland with respect to polyphenols compounds. By means of TLC and HPLC analyses we were able to detect several flavonoids: baicalein, luteolin, wogonin, apigenin, quercetin and kaempferol as well as ellagic and p-coumaric acids. From this study we can conclude a satisfactory quality of the crude drug from acclimated plants.