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Salix myrtilloides L. is an endangered species whose western limit of range runs through Poland. The main aim of the study was to increase the knowledge on the ecology and biology of S. myrtilloides populations in the Polesie Lubelskie region (Eastern Poland) in order to create an effective protection program. An 80% decrease in its population was found in this area. Our study was conducted to identify the mechanisms responsible for the process of withdrawal of this species from its natural stands by determining whether the processes of generative reproduction (pollen viability and germinability, seed germination ability and dynamics) in the populations occur properly and by characterizing within- and among-population genetic diversity of S. myrtilloides, using ISSR (Inter-Simple Sequence Repeat) primers. The results confirmed that S. myrtilloides pollen viability was high (84.17±8.67), and so were the seed germination ability (76% of the total number of sown seeds/24 h) and dynamics. The within-population genetic diversity was quite high for this species and the inter-population genetic variation was of medium value (ΦPT=0.148). The condition of two populations, their genetic diversity and sex ratio as well as the correctness of the investigated reproductive cycle stages altogether give prospects for their survival. It seems to be very important to start conservation involving the reintroduction and reconstruction of S. myrtilloides populations in the Polesie Lubelskie region. The proposed method for restoration of S. myrtilloides resources would be possible if ex situ cultivation and in vitro methods were used.
Changes in habitat conditions in the area of Eastern Poland (Polesie Podlaskie), often associated with anthropopressure, cause a reduction in the number of locations and population size of valuable and rare bog plant species, including a Pleistocene boreal relict, Salix myrtilloides. The aim of this study was to determine the current conditions of occurrence of this species based on the physico-chemical parameters of peatland piezometric groundwater. The results confirm the declining trend in the number of locations and abundance of this species in the past few decades, but at the same time they confirm the wide range of amplitude of the many investigated factors. Among the groundwater parameters studied, the low level of nitrogen fractions, phosphorus fractions, cations, and DOC can be considered to be a set of conditions promoting the preservation of the Salix myrtilloides population. The higher concentrations of phosphates, sulfates, Na, Ca, and Mg as well as higher pH and electrolytic conductivity can be considered to be presumably unfavorable. The lack of significant differences in the values of the piezometric water factors investigated for the sites with different population sizes of the studied species requires the identification of other limiting factors and the implementation of programs for its active protection in Poland.
Present status of a postglacial relict – Salix myrtilloides L. was investigated in north central Poland at 27 localities. Consequently 3 sites were confirmed, 4 were not confirmed, and the remaining 20 were considered to be extinct. In comparison to the historical distribution, ca. 74% of the sites existing 50 years ago do not exist today in the studied region. The confirmed populations were found near Oporówek (ca. 1600 stems), Rakowiec (ca. 100) and Bobrowo (15). At three sites confirmed in this study, the existence of vigorous hybrids between S. myrtilloides and S. aurita L. (S. ×onusta Besser) were observed. The most important threats to Salix myrtilloides L. populations in the studied region were assessed to be hybridization with other commonly occurring willow species, site isolation and habitat destruction. Natural habitat protection with adequate buffer zone and gene pool protection in a form of plantations was suggested as a conservation strategy. As an alternative strategy active protection ‘insitu’ was proposed based on cutting competitive common species of willows.
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