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Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.

100%

Acta Biologica Cracoviensia. Series Botanica

2017

tom 59

nr 2

Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

Responses in ion leakage of wild sugarcane [Saccharum spontaneum L.] clones to enhanced ultraviolet-B radiation under field conditions

84%

Yuan L., Yangun Z., Yongmei H., Haiyan C., Jianjun C., Fusheng L., Lilian H.

Acta Physiologiae Plantarum

2006

tom 28

nr 5

Field studies were conducted to determine the potential for alterations in ion leakage and the intraspecific variation in ion leakage sensitivity of 20 wild sugarcane clones (Saccharum spontaneum L.) to enhanced ultraviolet-B (UV-B, 280-315nm) radiation in two consecutive years. The clones were collected from original sites with different altitude (from 0 to 1650 m) and latitude (from 18-37 oN). The supplemental UV-B radiation was 5.00 kJ m-2, simulating a depletion of 20 % stratospheric ozone. Across all clones tested in the present study, a significant change (P<0.01 or 0.05) in ion leakage for 11 in tillering, 14 in elongation and 15 in flowering in 2003, and for 9 in tillering, 5 in elongation and 5 in floweing in 2004 were observed. In general, intraspecific responses of ion leakage of wild sugarcane clones to enhanced UV-B radiation existed under field conditions for two consecutive years, alt though intraspecific difference in 2003 was more obvious than that in 2004. Wild sugarcane clones originating from lower latitude or high elevation were not necessarily the more tolerant to enhanced UV-B radiation.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

1 Fusheng L.

1 Haiyan C.

1 Jianjun C.

1 Lilian H.

1 Yangun Z.

1 Yongmei H.

1 Yuan L.

1 2017

1 2006

Wyniki wyszukiwania

Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.

Responses in ion leakage of wild sugarcane [Saccharum spontaneum L.] clones to enhanced ultraviolet-B radiation under field conditions