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Genotypic diversity, antimicrobial resistance and screening of Vibrio cholerae molecular virulence markers in Vibrio alginolyticus strains recovered from a Tunisian Ruditapes decussatus hatchery

100%

Mechri B., Medhioub A., Medhioub M. N., Aouni M.

Polish Journal of Microbiology

2013

tom 62

nr 3

In this study, a total of 54 Vibrio alginolyticus strains were analyzed. The isolates were recovered from different compartments of the Ruditapes decussatus hatchery in the National Institute of Marine Sciences and Technologies, Monastir, Tunisia. All isolates were biochemically identified (API 20E and API ZYM strips), characterized by amplification of the Hsp-40 gene polymerase chain reaction (PCR) and analyzed by enterobacterial repetitive intergenic consensus (ERIC)-based genotyping to evaluate genetic relationship between the isolated strains. We also looked for the presence of ten V. cholera virulence genes (toxRS, toxR, toxT, toxS, tcpP, tcpA, ace, vpi, zot and ctxA) in the genomes of Vibrio isolates. The antibiotics susceptibility, exoenzymes production and in vitro cytotoxic activitiy against HeLa cell line were also carried out for all tested bacteria. Most of V. alginolyticus isolates showed significant antimicrobial resistance rates to at least ten antibacterial agents. For most isolates, the minimum inhibitory concentration (MIC) data showed that tetracyclin and streptomycin were the most effective antibiotics. Construction of the phylogenetic dendogram showed that studied isolates were in general genetically heterogeneous; however some Vibrio strains were present in different structures of the R. decussatus hatchery. The V. cholerae virulence genes investigation showed a wild distribution of toxS (49/54), toxR (45/54) and toxT (22/54) genes among V. alginolyticus strains isolated from the R. decussatus rearing system. Cytotoxic effects of several Vibrio extracellular products (28/54) were also observed on HeLa cells.

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Effect of transplantation on heavy metal concentrations in commercial clams of Lake Timsah, Suez Canal, Egypt

80%

Gabr H. R., Gab-Alla A. A.-F. A.

Oceanologia

2008

tom 50

nr 1

83-93

Ruditapes decussatus and Venerupis pullastra are commercially fished clams with a wide distribution in the shallow inshore waters of Lake Timsah, Egypt. They are usually contaminated with heavy metals. Consumption of such contaminated clams can pose a public health risk. To minimize this risk, therefore, the clams should be removed from the contaminated waters and transferred to an approved area to reduce the high levels of metals before being marketed. The aim of this work was to study the effect of transplantation on levels of heavy metals (Fe, Mn, Zn, Cu,Ni, Co,Cd,Pb) in these clams. The clams were removed from their polluted site and transplanted to a relatively clean area for a period of 120 days. Although the salinity at the transplantation site was higher than at the polluted site, it was stable and did not appear to have any adverse effect on clam growth. Heavy metals were analysed in the water, sediment and clam tissues from both the polluted and the transplantation sites. Although in both species transplantation evidently reduced heavy metal levels, these still exceeded the maximum permissible levels laid down by the WHO (1982).

Ograniczanie wyników

1 Oceanologia

1 Polish Journal of Microbiology

1 Aouni M.

1 Gab-Alla A. A.-F. A.

1 Gabr H. R.

1 Mechri B.

1 Medhioub A.

1 Medhioub M. N.

1 2013

1 2008

Wyniki wyszukiwania

Genotypic diversity, antimicrobial resistance and screening of Vibrio cholerae molecular virulence markers in Vibrio alginolyticus strains recovered from a Tunisian Ruditapes decussatus hatchery

Effect of transplantation on heavy metal concentrations in commercial clams of Lake Timsah, Suez Canal, Egypt