Wyniki wyszukiwania

1

Development of an effective method of selective RNAi-induced gene silencing in legumes

100%

Grabowska B., Figlerowicz M.

Polish Journal of Natural Sciences. Supplement

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2003

|

tom 01

2

The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors

88%

Ferrari R., Dieci G.

Cellular and Molecular Biology Letters

|

2008

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tom 13

|

nr 1

112-118

Transcription reinitiation by RNA polymerase (Pol) III proceeds through facilitated recycling, a process by which the terminating Pol III, assisted by the transcription factors TFIIIB and TFIIIC, rapidly reloads onto the same transcription unit. To get further insight into the Pol III transcription mechanism, we analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised.

3

Effects of the hepatocarcinogen 2-aminofluorene on RNA synthesis in rat liver

88%

Widlak P., Rzeszowska-Wolny J.

Acta Biochimica Polonica

|

1992

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tom 39

|

nr 1

4

Changes in the activity of transport of newly synthesized RNA from nucleus into cytoplasm during interphase

75%

Kwiatkowska M., Maszewski J.

Folia Histochemica et Cytobiologica

|

1985

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tom 23

|

nr 3

5

Clonal life and temperature resistance in Paramecium primaurelia

75%

Ramoino P., Delmonte Corrado M. U., Crippa Franceschi T.

Acta Protozoologica

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1988

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tom 27

|

nr 1

6

In vitro transcription of class III genes

75%

Glanc P., Galbas M., Dullin P.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

7

Tryptic digestion of peptides corresponding to modified fragments of human growth hormone-releasing hormone

75%

Witkowska E., Orlowska A., Izdebski J.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 1

The objective of this study was to examine the degradation of short peptides corresponding to modified fragments of human growth hormone-releasing hormone by trypsin. Six analogues of pentapeptide 9-13 of human growth hormone-releasing hormone containing homoarginine, ornithine, glutamic acid, glycine, leucine or phenylalanine residue in position 11, two analogues of hexapeptide 8-13 of human growth hormone-releasing hormone and two analogues of heptapeptide 7-13 of human growth hormone-releasing hormone containing homoarginine or glycine residue in position 11 were obtained. The peptides were subjected to digestion by trypsin and the course of reaction was monitored using HPLC. It was found that the rate of hydrolysis of the Lys12-Val13 peptide bond depends on the amino-acid residue preceding Lys12 . The extension of the peptide chain towards the N-terminus by introduction of consecutive amino-acid residues corresponding to the human growth hormone-releasing hormone sequence accelerates the hydrolysis process. These results may be of assistance in designing new analogues of human growth hormone-releasing hormone, more resistant to the activity of proteolytic enzymes.

8

Effect of lead on the transcriptional activity of chromatin in lupin roots

75%

Schneider J., Kucharczyk A.

Biological Bulletin of Poznań. Supplement

|

1996

|

tom 33

9

Functional and structural heterogeneity of nucleoli: the dependence of the activity of transport of newly synthesized rRNA on nucleolar size and phase of the cell cycle

63%

Kwiatkowska M., Maszewski J.

Folia Histochemica et Cytobiologica

|

1985

|

tom 23

|

nr 3

10

The effect of juvenile hormone on RNA synthesis in the silk glands of Galleria mellonella

63%

Michalik J., Sehnal F.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1989

|

tom 37

|

nr 7-9

11

Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium

63%

Wolun-Cholewa M., Szymanowski K., Andrusiewicz M., Szczerba A., Warchol J. B.

Folia Histochemica et Cytobiologica

|

2010

|

tom 48

|

nr 1

148-152

12

The effect of protein phosphorylation on in vitro class III gene transcription

63%

Glanc P., Galbas M., Dullin P.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

13

In vitro effect of methisoprinol on salmonid rhabdoviruses replication

63%

Siwicki A. K., Pozet F., Morand M., Kazun B., Trapkowska S.

Bulletin of the Veterinary Institute in Puławy

|

2002

|

tom 46

|

nr 1

14

PML shuttles between nuclear bodies and the cytoplasm

63%

Stuurman N., Floore A., Middelkoop E., Van Driel R., De Jong L.

Cellular and Molecular Biology Letters

|

1997

|

tom 02

|

nr 2

Nuclear bodies are electron dense, spherical structures with a diameter between 0.2 and 1.0 µm. The function of these nuclear domains is unclear. One class of nuclear bodies contains the tumor suppressor protein PML. Besides in the nucleus, a small number of PML-containing structures of about the same size as nuclear bodies is also present in the cytoplasm. We have investigated whether PML is transported from the nucleus to the cytoplasm and/or vice versa. To this end we injected the PML-specific mAb 5E10 into the cytoplasm of living cells. Subsequently, we monitored translocation of the antibody across the nuclear envelope by indirect immunofluorescent microscopy. It is well known that antibodies in the cytoplasm of living cells do not enter the nucleus, unless as a complex with a karyophilic protein. We observed accumulation into PML-containing nuclear bodies of 5E10 microinjected into the cytoplasm. Control rabbit IgG and a mAb specific for lamin B2 were not translocated to the nucleus. All nuclear PML bodies were labeled simultaneously by 5E10 with gradually increased intensity in time. Labeling of PML-containing nuclear bodies by 5E10 microinjected into the cytoplasm was not affected by inhibition of protein synthesis. These results suggest that the 5E10 antigen PML shuttles between the nucleus and cytoplasm, indicating that nuclear bodies are dynamic structures.

15

Effect of calcium and toxic metals on RNA synthesis and transport in cortex cells of root meristem of Pisum sativum L.

63%

Gabara B., Krajewska M.

Polish Journal of Environmental Studies

|

1997

|

tom 06

|

nr 3

Experiments with 3H uridine incorporation showed that a 144-hr treatment with cadmium, chromium or lead, each at a concentration of 10-4 M, as well as the addition of calcium (10 -3 M), affected RNA in the nucleolus, nucleoplasm and cytoplasm in cortex cells of pea root meristem. Disturbances in RNA transport (especially from the nucleolus to the cytoplasm) appearing due to toxic metals and calcium suggest that this RNA was mostly of nucleolar origin.

16

Lymphocyte phenotyping of renal transplanted patients under two different immunosuppressive protocols

63%

Tzanno-Martins C., Araujo P., Orii N., Duarte A. J. S., Paula F. J., Duley J. A., Nahas W., Arap S., Ianhez L. E., Chocair P. R.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

17

The properties of plant [Cucurbita pepo] nuclear matrix preparations are strongly affected by the preparation method

63%

Rzepecki R.

Cellular and Molecular Biology Letters

|

2000

|

tom 05

|

nr 2

Nuclear matrices from White bush (Cucurbita pepo var. patisoniana) cell nuclei were isolated. Three different preparation methods were used. The methods were: I- the method of Berezney and Coffey [1] involving extraction of cell nuclei with 2M NaCl and Triton X-100 (called the “High Salt” method); II- the same with pre-treatment of cell nuclei with 0.5 mM CuSO4 (stabilisation step); III- the method of Mirkovitch et al. [2] involving lithium diiodosalicylate (LIS) extraction (called the “LIS” method). Each of the three methods was used in three variants of nucleic acid removal: restriction enzymes, endogenous nucleases and DN-ase I with RN-ase A digestion. Nuclear matrices were analysed for protein and DNA content, residual RNA and DNA synthesis activity, endonucleolytic activity and specific SAR DNA binding properties. The lowest protein and DNA content and endonucleolytic activity was found in nuclear matrices isolated by the “High Salt” method. It also had the lowest RNA and DNA synthesis and endonucleolytic activity. The highest protein and DNA content, and RNA and DNA synthesis and endonucleolytic activity was found in nuclear matrices isolated by the “LIS” method. When exogenous SAR DNA binding activity was compared, the highest was found in nuclear matrices isolated by the “High Salt” method while the lowest was in the “LIS” method preparation. Nuclear matrices isolated by the “High Salt” method with a stabilisation step always displayed average values of assayed parameters. These data indicate that the biological residual properties of a nuclear matrix preparation strongly depend on the method used.

18

Effect of DNA-interacting drugs on phage T7 RNA polymerase

63%

Piestrzeniewicz M., Studzian K., Wilmanska D., Plucienniczak G., Gniazdowski M.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 1

9-Aminoacridine carboxamide derivatives studied here form with DNA intercalative complexes which differ in the kinetics of dissociation. Inhibition of total RNA synthesis catalyzed by phage T7 and Escherichia coli DNA-dependent RNA polymerases correlates with the formation of slowly dissociating acridine-DNA complex of time constant of 0.4-2.3 s. Their effect on RNA synthesis is compared with other ligands which form with DNA stable complexes of different steric properties. T7 RNA polymerase is more sensitive to distamycin A and netropsin than the E. coli enzyme while less sensitive to actinomycin D. Actinomycin induces terminations in the transcript synthesized by T7 RNA polymerase. Despite low dissociation rates of DNA complexes with acridines and pyrrole antibiotics no drug dependent terminations are observed with these ligands.

19

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Synthesis and fungicidal activity of substituted isoxazolecarboxamides

51%

Gucma M., Golebiewski W., Michalczyk A.

Pestycydy

|

2010

|

nr 1-4

Several new 3-substituted isoxazolecarboxamides have been prepared from aromatic and aliphatic aldehydes. A key step was a 1,3-dipolar cycloaddition of nitrile oxides to ethyl acrylate or methacrylate esters. Some of the amides showed high fungicidal activities against Alternaria alternate, Botrytis cinerea, Rhizoctonia solani, Fusarium culmorum, and Phytophthora cactorum strains.

20

HPLC-analysis of low amounts of methotrexate and its polyglutamates in red blood cell lysates after low-dose methotrexate maintenance treatment for childhood acute lymphoblastic leukaemia

51%

Ter Riet P. G. J. H., Brouwer C., De Abreu R. A., Bokkerink J. P. M., Trijbels F. J. M.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

Ograniczanie wyników

Development of an effective method of selective RNAi-induced gene silencing in legumes

The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors

Effects of the hepatocarcinogen 2-aminofluorene on RNA synthesis in rat liver

Changes in the activity of transport of newly synthesized RNA from nucleus into cytoplasm during interphase

Clonal life and temperature resistance in Paramecium primaurelia

In vitro transcription of class III genes

Tryptic digestion of peptides corresponding to modified fragments of human growth hormone-releasing hormone

Effect of lead on the transcriptional activity of chromatin in lupin roots

Functional and structural heterogeneity of nucleoli: the dependence of the activity of transport of newly synthesized rRNA on nucleolar size and phase of the cell cycle

The effect of juvenile hormone on RNA synthesis in the silk glands of Galleria mellonella

Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium

The effect of protein phosphorylation on in vitro class III gene transcription

In vitro effect of methisoprinol on salmonid rhabdoviruses replication

PML shuttles between nuclear bodies and the cytoplasm

Effect of calcium and toxic metals on RNA synthesis and transport in cortex cells of root meristem of Pisum sativum L.

Lymphocyte phenotyping of renal transplanted patients under two different immunosuppressive protocols

The properties of plant [Cucurbita pepo] nuclear matrix preparations are strongly affected by the preparation method

Effect of DNA-interacting drugs on phage T7 RNA polymerase

Synthesis and fungicidal activity of substituted isoxazolecarboxamides

HPLC-analysis of low amounts of methotrexate and its polyglutamates in red blood cell lysates after low-dose methotrexate maintenance treatment for childhood acute lymphoblastic leukaemia