The inflorescences of yellow everlasting collected from twenty two natural sites in the middle part of the Bug river valley and two in the Puszcza Knyszyńska forest were compared in respect to the content and composition of flavonoids and phenolic acids. Genetic distances among populations, evaluated by RAPD markers, fluctuated from 0.0968 to 0.4855. There were no dependences between genetic relationships and geographical distributions of populations, as well as between their genetic relationships and chemical compositions. The total content of flavonoids ranged from 0.15 to 0.70% and phenolic acids from 0.45 to 1.52%. As a result of the HPLC analysis no flavonoid compounds were identified, with isosalipurposide as a dominant constituent. The chlorogenic, caffeic, ferulic and rosmarinic acids were also detected.
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