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Molecular mapping and location of QTLs for drought-resistance traits in indica rice (Oryza sativa L.) lines adapted to target environments

100%

Gomez S. M., Boopathi N. M., Kumar S. S., Ramasubramanian T., Chengsong Z., Jeyaprakash P., Senthil A., Babu R. C.

Acta Physiologiae Plantarum

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2010

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tom 32

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nr 2

Drought is a major limitation for rice production in rainfed ecosystems. Identifying quantitative trait loci (QTLs) linked to drought resistance provides opportunity to breed high yielding rice varieties suitable for drought-prone areas. Although considerable efforts were made in mapping QTLs associated with drought-resistance traits in rice, most of the studies involved indica × japonica crosses and hence, the drought-resistance alleles were contributed mostly by japonica ecotypes. It is desirable to look for genetic variation within indica ecotypes adapted to target environment (TE) as the alleles from japonica ecotype may not be expressed under lowland conditions. A subset of 250 recombinant inbred lines (RILs) of F₈ generation derived from two indica rice lines (IR20 and Nootripathu) with contrasting drought-resistance traits were used to map the QTLs for morpho-physiological and plant production traits under drought stress in the field in TE. A genetic linkage map was constructed using 101 polymorphic PCR-based markers distributed over the 12 chromosomes covering a total length of 1,529 cM in 17 linkage groups with an average distance of 15.1 cM. Composite interval mapping analysis identified 22 QTLs, which individually explained 4.8–32.2% of the phenotypic variation. Consistent QTLs for drought-resistance traits were detected using locally adapted indica ecotypes, which may be useful for rainfed rice improvement.

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Interval mapping of QTLs controlling some morphological traits in pea

100%

Irzykowska L., Wolko B., Swiecicki W. K.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

A linkage map of pea was constructed based on a 104 RIL population derived from the cross combination Wt10245 x Wt11238. The map, which consisted of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of the QTLs controlling the stem length and internode number of pea. In the characterization of a given QTL, we included an identification of its position with reference to the ﬂanking markers, an estimation of the part of variance explained by it, and a determination of gene action. Six QTLs per trait were identified as demonstrating linkage to ten intervals on five linkage groups. As many as seven QTLs inﬂuencing the analysed traits were mapped on linkage group II, indicating the important role of this region of the pea genome in plant height control.

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Effect of salt on growth of rice landraces in Vietnam

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Anh L. H., Hue H. T., Quoc N. K., Nghia L. T., Trung K. H., Trung T. N., Trang D. H., Xuan T. D., Khanh T. D.

International Letters of Natural Sciences

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2016

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tom 59

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Detection of quantitative trait loci affecting carcass traits and internal organs on chromosome 3 in an F2 intercross of Japanese quail

84%

Charati H., Koshkoiyeh A. E., Ori R. J., Moradian H., Mehrgardi A. A.

Animal Science Papers and Reports

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2014

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tom 32

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nr 4

Molecular technologies based on markers indicating differences among individuals at the DNA level can play an important role in genetic improvement of carcass traits through marker or gene assisted selection. The purpose of this study was to map quantitative trait loci (QTL) of chromosome 3 affecting carcass traits on Japanese quail using microsatellite markers. Two white and wild strains of Japanese quail were crossed reciprocally and the F1 generation was created. The F2 generation was generated by intercrossing F1 birds. Phenotypic data including weights of hot and cold carcasses,carcass parts and internal organs were collected from 422 F2 birds. The total mapping population (472 birds) was genotyped for microsatellite markers. QTL analysis was performed using the least squares regression interval mapping method. Significant QTL were identified for hot and chilled carcass weights, liver weight, head percentage, uropygial gland percentage, intestine percentage, ovary weight, uropygial gland weight, pancreas percentage (0-36 cM with an additive effect),proventriculus percentage, head weight (6-20 cM with a dominance effect), and gizzard percentage (0 cM with an imprinting effect).

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Mapping QTLs for alpha-amylase activity in rye grain

84%

Masojc P., Milczarski P.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

Genetic control of a-amylase activity in rye grain was investigated by QTL mapping/based on DS2 x RXL10 intercross consisting of 99 F₅₋₆ families propagated at one location during four vegetation seasons. A wide range of variation in α-amylase activity and transgression effects were found among families and parental lines. This variation was shown to be determined in 40.1 % by 7 significant (LOD score not less than 2.5) and 2 putative QTLs (2 < LOD < 2.5) distributed on all rye chromosomes except 4R. Two significant QTLs located on 3RL and 5RL chromosome arms were expressed each year. The third significant QTL was detected in three years (1RL). The other four significant QTLs (2RL, 5RS, 6RL, 7RL) were found in one year of study. The number and composition of QTLs were specific for a given year varying from three to six. QTLs were not correlated with isoenzyme polymorphisms at the structural α-Amyl loci. A QTL associated with a region containing the α-Amy3 locus was detected on chromosome 5RL. Both high- and low-activity QTL alleles were found in each parental line, which explains the appearance of transgressive recombinants in the segregating population.

Ograniczanie wyników

Molecular mapping and location of QTLs for drought-resistance traits in indica rice (Oryza sativa L.) lines adapted to target environments

Interval mapping of QTLs controlling some morphological traits in pea

Effect of salt on growth of rice landraces in Vietnam

Detection of quantitative trait loci affecting carcass traits and internal organs on chromosome 3 in an F2 intercross of Japanese quail

Mapping QTLs for alpha-amylase activity in rye grain