The study has determined the composition of dissolved organic matter in Luvisols, Fluvisols and Histosols using spectroscopic (FTIR) and chromatographic (HPLC and Py-GCMS) methods. It has been found that aliphatic hydrocarbons (linear) containing from 4 to 12 atoms of carbon constitute the dominant group of compounds included in the dissolved organic matter (DOC). The preparations isolated from Histosols and Luvisols demonstrated a higher proportion of hydrophobic fraction with a longer retention time probably containing more compounds with long-chain aliphatic and simple aromatic structure than the DOC of Fluvisols. The differences in infrared spectra are evident particularly in the wave number between 1650–1030 cm⁻¹. The DOC of Histosols is richer in aromatic compounds (range 1620 cm⁻¹) but the DOC of Luvisols and Fluvisols is richer in alkene chains and hydroxyl (OH) and methoxy (OCH₃) groups. The results showed differences in the composition of the DOM across the soils, caused their genesis.