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1

Identification and differentiation of zoonotic Escherichia coli strains isolated from healthy sheep, by molecular methods

100%
Nawrotek P., Fijalkowski K., Czernomysy-Furowicz D., Michalek E., Solecka A.
Advances in Agricultural Sciences
|
2011
|
tom 14
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nr 1-2
2

The immune response against gastrointestinal nematodes in naturally infected Polish Wrzosowka sheep. I. The serum IgG response to Haemonchus contortus in ewes over three grazing seasons

80%
Moskwa B.
Acta Parasitologica
|
1999
|
tom 44
|
nr 4
Faecal nematode egg counts and IgG activity to Haemonchus contortus were followed in naturally infected Polish Wrzosówka ewes. The egg counts were overdis- persed; most individuals had relatively low egg counts but a small proportion had high counts. Egg production followed a different pattern each grazing season. Larvae cultured from eggs in 1996 were predominantly H. contortus and Trichostrongylus spp. In 1997 the recoveries were largely Trichostrongylus spp. In 1998, larval recoveries were mainly Trichostrongylus spp. and Teladorsagia spp. There were no discernible patterns in the composition of the nematode population within each grazing season. Egg counts in different months were moderately repeatable, indicating that animals tended to maintain their rankings over time. The repeatabilities rose during the grazing season but declined with increasing intervals between sample dates. IgG activity against a somatic extract of adult H. contortus was higher than activity against a preparation from excretory/secretory antigens but the two responses were very strongly correlated. The repeatability of IgG activity at monthly intervals was higher than the repeatability of faecal egg counts. Animals with higher than average IgG activity had significantly lower than average egg counts but only in the last two years of the study. A combination of egg counts and antibody responses may be better at identifying resistant animals than either method used in isolation, but more research is necessary to determine why the association between antibody and egg counts varies in different years.
3

The immune response against gastrointestinal nematodes in naturally infected Polish Wrzosowka sheep. III. Comparison of IgG response against different antigen preparations of adult Haemonchus contortus in young ewes

80%
Moskwa B.
Acta Parasitologica
|
2000
|
tom 45
|
nr 1
The association between faecal egg counts and serum IgG response against somatic (SAg) and two excretory/secretory antigens (ESAg-4h, ESAg-24h) obtained from adult Haemonchus contortus was monitored over one season in young Polish Wrzosówka ewes naturally infected with gastrointestinal nematodes. There was a considerable variation in faecal egg counts and in the proportion of larvae from each species recovered from faeces at different months. Very similar IgG responses against the three antigen preparations were observed; the differences among months were significant. The distribution of antibody among young ewes was unimodal and positively skewed. The repeatabilities of IgG responses against SAg and ESAg-24h were all positive and statistically significant, although repeatabilities against ESAg-4h were of border line significance. The correlation coefficients among IgG responses tended to increase in the second half of season. There was a significant association between increased IgG responses to somatic antigens and reduced faecal egg counts. The combination of antibody responses against different antigen preparations of H. contortus and faecal egg counts appear better at identifying resistant Polish Wrzosówka sheep than either method used in isolation.
4

The immune response against gastrointestinal nematodes in naturally infected Polish Wrzosowka sheep. II. The serum IgG response to nematode somatic antigens in young ewes over three grazing seasons

80%
Moskwa B.
Acta Parasitologica
|
1999
|
tom 44
|
nr 4
A longitudinal study in young Polish Wrzosówka ewes, naturally infected with gastrointestinal nematodes, was carried out to examine the association between parasite-specific IgG antibody and faecal egg counts. IgG activity varied among the young ewes in each sampling month. The highest individual values were measured in the second half of the each season, when the overall means were highest. The distribution of IgG activity among individuals was unimodal with a positive skew. This study appears the first to examine the distribution, among animals and over time, of IgG responses to natural mixed infection. Serum IgG responses were repeatable and significantly associated with reduced nematode faecal egg counts. A combination of faecal egg counts and IgG responses can be used to identify animals with increased resistance to nematode infection in this breed of sheep.
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