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In 1993–1997, 990 pupae of diamondback moth (Plutella xylostella L.) were collected from nine different varieties of Brasica oleracea L., grown on experimental farm near Kraków. Only 234 moths (23.6%) were recovered from the cocoons. The most important factor reducing the population of DBM was parasitization (65.1%). It varied between the years of observation and oscillated from 60% to 90.3%. Sixhundred and fourty four specimens of parasitic wasps belonging to 11 species from families: Ichneumonidae (5 species), Braconidae (3 species), Pteromalidae (2 species) and Eulophidae (1 species) were reared out. In each year of observations the most abundant species among DBM parasitoids was Diadegma fenestralis Holmgr. as it constituted 71.4% of all of the wasps reared. In the years 1994 and 1996 with low abundance of diamondback moth, D. fenestralis was the only species parasitizing 66.7% and 89.5% of the pest larvae
Bacillus thuringiensis is a Gram-positive, aerobic, facultative anaerobic and endosporeforming bacterium. Different strains of this species have the ability to produce parasporal crystalline inclusions which are toxic to larvae of different insect orders and other invertebrates and cause rapid death of the host. To determine the importance of this species in microbial control, we collected native strains and studied their virulence on the diamondback moth, Plutella xylostella. More than 148 samples were collected from Alborz, Guilan and Mazandaran Provinces. Experimental samples, including soil samples from forests, fruit gardens, agricultural fields, diseased and dead larvae, were transferred to a laboratory in sterile plastic containers. For evaluating B. thuringiensis isolates virulence, a cabbage leaf dip method with 106 cell ⋅ ml–1 concentration of various Bt isolates was applied to diamondback moths. Larval mortality was recorded 72 h after treatment. Based on bioassay results, all isolates were classified into three high, medium and low virulence groups. Protein level characterization based on the SDS-PAGE gel analysis showed that two isolates from a high virulence group have proteins of high molecular masses of 121 and 109 kDa. Results revealed that there is a positive correlation between protein masses and virulence of isolates. In addition, this research introduced nine strains that are highly toxic to P. xylostella and would be valuable as insecticidal agents for controlling lepidopteran pests.
The three-dimensional conformation of Aea-TMOF (Aedes aegypti Trypsin Modulating Oostatic Factor), a decapeptide (YDPAPPPPPP) isolated from mosquito ovaries that inhibits the translation of many trypsin-like serine proteases, e.g. in mosquitoes, flies and lepidopterans, was used as a model for the synthesis of 10 aromatic and aliphatic organic acid and ester analogues. The organic TMOF analogues were tested against herbivorous pest lepidopterans and larval Ae. aegypti. The compounds administered to microtiter plates or to leaf disks caused mortality to mosquito larvae and the diamondback moth, Plutella xylostella, larvae 3-6 days after treatment. The surviving diamondback moth larvae were sluggish, immobile and stopped feeding. A biochemical analysis showed that in larval P. xylostella the trypsin activity was low and correlated with the observed mortality. No activity was found against Heliothis virescens when four of the synthetic compounds that affected P. xylostella and Ae. aegypti were tested, although the decapeptide, TMOF, does affect trypsin biosynthesis in H. virescens.
Cabbage (Brassica oleracea Linn.) is one of the important vegetables grown worldwide. Insect pests have been a major constraint to its production with some reported to have developed resistance to commonly available synthetic insecticides. In order to find alternative control for these pests, field experiments were conducted in the early seasons of 2011 and 2012, to evaluate the efficacy of three plant extracts, Tephrosia vogelli Hooks, Zingiber officinales Rosh and Lantana camara Spanish flag, applied at 5, 10 and 25 % w/v concentrations Delthamethrin (a synthethic insecticide) and an unsprayed plot were included as positive and negative controls respectively. Plots were arranged in a Randomized Complete Block Design (RCBD), with three replications. Mean Data over the two planting seasons were subjected to the analysis of variance and significant means were compared using the least significant difference (L.S.D) at P < 0.05. Z. officinales extract effectively control P. xylostella larvae at all concentrations with 25 % w/v gave the best yield of 0.50 kg/ha while T. vogelii and L. camara extracts were as effective as Deltamethrin at different concentrations and hours on diamondback-moth (P. xylostella) adult The present findings indicated that effective botanicals against Diamondback moth adult and larvae of cabbage can be formulated from extract of T. vogelii, L. camara and Z. officinales at 25 % concentration respectively.
Control failure of pests and selectivity of insecticides to beneficial arthropods are key data for the implementation of Integrated Pest Management (IPM) programs. Therefore, the aim of this study was to assess the control failure likelihood of Plutella xylostella and the physiological selectivity active ingredients to parasitoid Oomyzus sokolowskii (Hymenoptera: Eulophidae) and to predators Polybia scutellaris (Hymenoptera: Vespidae) and Lasiochilus sp. (Hemiptera: Anthocoridae). In bioassays, P. xylostella larvae and O. sokolowskii, P. scutellaris and Lasiochilus sp. adults were used. Concentration-mortality curves of six insecticides for P. xylostella were established. These curves were used to estimate the mortality of P. xylostella at the recommended concentration, in order to check a control failure of insecticides to this pest. Furthermore, the lethal concentration for 90% of populations (LC90) and the half of LC90 were used in bioassays with the natural enemies to determine the selectivity of these insects to insecticides. All tested insecticides showed control failure to P. xylostella, indicated by high LC90 and low estimated mortalities (less than 80%). The cartap insecticide was selective in half of LC90 to Lasiochilus sp. and moderately selective in LC90 and the half of LC90, to Lasiochilus sp. and P. scutellaris, respectively. Deltamethrin was moderately selective in the half of LC90 to predator Lasiochilus sp. Cartap, carbaryl, and deltamethrin reduced the mortality of Lasiochilus sp. in the half LC90. The results also showed that the insecticides methamidophos, carbaryl, parathion methyl and permethrin were not selective to any of the tested natural enemies. The role of insecticides in IPM systems of Brassica crops is discussed based on their control failures to P. xylostella and selectivity to their natural enemies.
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Migracje w świecie motyli

51%
Field trials were conducted in two localities: Skierniewice and Powiercie near Koło in 2007–2008 to evaluate the efficacy of DiPel DF containing Bacillus thuringiensis sub. kurstaki (54%) (dose: 0.5 and 1.0 kg/ha) in controlling the fuor species of pests: Pieris brassicae, Pieris rapae, Mamestra brassica, Plutella xylostella on white cabbage. The application of DiPel DF at two mentioned doses gave very good results in the control of four presented pests. It was stated a 100% mortality, 7 days after treatment with DiPel DF.
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