Pityrosporum pachydermatis was isolated from 28% of the samples collected from clinically positive cases of dermatomycosis in dogs. In two cases Pityrosporum was isolated together with dermatophytes. The aim of the investigations was to determine a frequency of P. pachydermatis infections in dogs with dermatomycosis and to evaluate the drug resistance of the isolated strains. Samples collected from dogs were cultivated on Sabouraud agar with actidion for 3 weeks at 30°C. In additional, microscopic examinations were carried out. The inoculated plates were read for fungal growth in 3-5 day intervals. After 3-4 days of incubation, small colonies of P. pachydermatis were observed although the incubation temperature was lower than that optimal for P. pachydermatis growth. Drug sensitivity was determined by the disc method and by ATB-Fungus Bio-Merieux Kit. It was found out that P. pachydermatis may be the only causative agent of derma- tomycoses and that it may also be isolated together with other dermatophytes (Microsporum, Trichophyton). The isolates appeared to be significantly sensitive to imidazole, low sensitive to 5-fluorocytosine, imidazolederivatives and nystatin.
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