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Screening and characterization of Fibrinolytic protease producing Bacillus circulans from mangrove sediments pitchavaram, South East Coast of India

100%

Sadeesh Kumar R., Rajesh R., Gokulakrishnan S., Subramanian J.

International Letters of Natural Sciences

2015

tom 01

Regulation and production of Fibrinolytic enzymes from bacterial sources especially from Bacillus strains has taken a leading role in the medical sciences for the treatment of cardiovascular disorders as it removes thrombus or clots adding to its significant role in curing human health issues saving millions. Significant progress has been made during the last few years on the studies of fibrinolytic enzymes in identifying, cloning, purification, characterization and overproduction of these for commercialization in medical sciences and in fields like detergents development. Production of fibrinolytic enzyme from Bacillus circulans was done using Nutrient broth medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous with other species of same genus, as examined by SDS−PAGE and sephadex G-75 column chromatography. The enzyme had an optimal pH of 7-12, an optimal temperature of 50 °C, for fibrin hydrolysis. The molecular mass estimated by gel filtration was 24 to36 KDa. Further studies for characterization and structural elucidation are necessary for their medicinal applications and molecular biological characteristics.

Screening of Actinomycetes from mangrove ecosystem for L-asparaginase activity and optimization by response surface methodology

80%

Usha R., Mala K. K., Venil C. K., Palaniswamy M.

Polish Journal of Microbiology

2011

tom 60

nr 3

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenotypic characteristics, strain KUA106 was identified as Streptomyces parvulus KUA106. The optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Streptomycetes parvulus. Four medium factors were screened from eleven medium factors by Plackett-Burman design experiments and subsequent optimization process to find out the optimum values of the selected parameters using central composite design was performed. Asparagine, tryptone, d))extrose and NaCl components were found to be the best medium for the L-asparaginase production. The combined optimization method described here is the effective method for screening medium factors as well as determining their optimum level for the production of L-asparaginase by Streptomycetes parvulus KUAP106.

Ograniczanie wyników

1 International Letters of Natural Sciences

1 Polish Journal of Microbiology

1 Gokulakrishnan S.

1 Mala K. K.

1 Palaniswamy M.

1 Rajesh R.

1 Sadeesh Kumar R.

1 Subramanian J.

1 Usha R.

1 Venil C. K.

1 2015

1 2011

Wyniki wyszukiwania

Screening and characterization of Fibrinolytic protease producing Bacillus circulans from mangrove sediments pitchavaram, South East Coast of India

Screening of Actinomycetes from mangrove ecosystem for L-asparaginase activity and optimization by response surface methodology