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The bacterial strains, Paenibacillus sp. (AY952466) and Bacillus cereus (DQ002384), have proven capacity to degrade lignin and pentachlorophenol. In the present study, both strains were screened at different concentrations of phenol on mineral salt agar medium in the presence of glucose. At optimized condition (pH 7.5 ± 0.2,37 ± 1°C, 120 rpm, 1% glucose w/v), it was observed that both Paenibacillus sp., B. cereus and its mixed culture degraded phenol (500 mg/l) up to 53.86%, 91.63% and 67.76% within 168 h of incubation, respectively. Phenol degradation was routinely monitored spectrophotometrically and further confirmed by HPLC. Catechol and 2-hydroxy muconic semialdehyde were identified as intermediate products from degraded samples using GC-MS. It was also observed that, in the absence of glucose, bacterial strains were unable to utilize phenol indicating the phenomenon of co-metabolism.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 09
s.600-603,rys.,tab.,bibliogr.
The paper characterizes Paenibacillus genera as well as species occurring in milk and its production environment. Paenibacillus bacteria are important part of environment. They are isolated from soil, rhizosphere especially, water, infected larvae, pasteurized food, cow manure, and municipal waste. The importance of these bacteria arises from their ability to form spores that survive the heat treatment of raw milk (pasteurization). Paenibacillus bacteria can enter raw milk during milking, from the bedding materials, feed, or teat surfaces, during the transfer of milk to the tank car, and as post-pasteurization recontamination in the dairy plant. The spores of Paenibacillus bacteria can germinate in cold storage, and vegetative cells are able to multiply in pasteurized milk stored at low temperatures and spoil it. So far, there are no Polish data on the presence of Paenibacillus bacteria or their spores (either their number or range) in raw milk and its production environment, or in pasteurized milk and milk products. New techniques, such as microfiltration and bactofugation, prove effective in eliminating psychotrophic bacteria spores (i.e. Paenibacillus bacteria) from raw milk.
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