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Phosphorylation of yeast ribosomal proteins by CKI and CKII in the presence of heparin

100%

Wojda I., Cytrynska M., Frajnt M., Jakubowicz T.

Acta Biochimica Polonica

1999

tom 46

nr 1

We have found that heparin has a different effect on Trichosporon cutaneum ribosomal protein phosphorylation by CKI and by CKII. In the presence of heparin, modification of 13 kDa, 19 kDa and 38 kDa proteins catalyzed by CKII was inhibited, while in the case of CKI, in addition to protein of 15 kDa, phosphorylation of 20 kDa and 35 kDa proteins was detected. It was also found that, in the presence of heparin, phosphorylation of P proteins (13 kDa and 38 kDa) by ribosome-bound protein kinases was inhibited. Moreover at the same conditions modification of 40 kDa protein was observed in all four yeast species tested.

Extraribosomal function of the acidic ribosomal P1-protein YP1alpha from Saccharomyces cerevisiae

100%

Tchorzewski M., Boldyreff B., Grankowski N.

Acta Biochimica Polonica

1999

tom 46

nr 4

The yeast acidic ribosomal P-proteins YP1α, YP1β, YP2a and YP2b were studied for a possible transactivation potential beside their ribosomal function. The fusions of P-proteins with the GAL4 DNA-binding domain were assayed toward their transcriptional activity with the aid of reporter genes in yeast. Two of the P-proteins, YP1α and YP1β, exhibited transactivation potential, however, only YP1α can be regarded as a potent transactivator. This protein was able to transactivate a reporter gene associated with two distinct promoter systems, GAL1 or CYC1. Additionally, truncated proteins of YP1α and YP1β were analyzed. The N-terminal part of YP1α fused to GAL4-BD showed transactivation potential but the C-terminal part did not. Our results suggest a putative extraribosomal function for these ribosomal proteins which consequently may be classified as "moonlighting" proteins.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Boldyreff B.

1 Cytrynska M.

1 Frajnt M.

1 Grankowski N.

1 Jakubowicz T.

1 Tchorzewski M.

1 Wojda I.

2 1999

Wyniki wyszukiwania

Phosphorylation of yeast ribosomal proteins by CKI and CKII in the presence of heparin

Extraribosomal function of the acidic ribosomal P1-protein YP1alpha from Saccharomyces cerevisiae