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Ostertagia circumcincta: surface and cuticular proteins and glycoproteins isolated by biotin-streptavidin affinity chromatography

100%

Wedrychowicz H.

Acta Parasitologica

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1994

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tom 39

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nr 1

Surface proteins and glycoproteins of ex- sheathed L3 (XL3), L4 and adults were identified by labelling live worms with NHS-SS-biotin which labels primary amine groups and biotin hydrazide (BH) which introduces biotin into sugar residues. After labelling and extensive washing, worms were homogenised in low-osmotic-strength buffer (TBS) and subjected to sequential extractions with SDS and SDS + 2-Me containing buffers. Labelled proteins released from the worms by these procedures were analysed by SDS-PAGE followed by Western blotting and probing with AP-streptavidin. Western blot analysis of biotinylated cuticular proteins from parasitic stages of O. circumcincta revealed stage specific differences in the labelling patterns of the cuticular proteins and glycoproteins. Developmental differences in cuticular components were apparent in the variety of MW of the components seen in Western blots although some components identified in Western blots appeared to be conserved (78 and 45 kDa). Two to six polypeptides were recognised in XL3 surface extracts by serum from sheep vaccinated with adult nematode somatic proteins. The surface of fourth stage larvae showed less cross-reactivity. Interestingly, the molecular weights of polypeptides recognised by anti-adult serum in streptavidin affinity isolated XL3 cuticular proteins were different from those recognised in adult cuticular proteins, which suggested that the cross-reacting epitopes were located on different proteins.

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Surface antigens of exsheathed infective larvae of Ostertagia circumcincta

100%

Wedrychowicz H., Holmes P. H., Tait A.

Acta Parasitologica

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1994

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tom 39

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nr 3

3

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The effect of anthelminthic treatment or immunisation of ewes during pregnancy on periparturient rise in ewes and liveweight gains of lambs

63%

Stankiewicz M., Hadas E.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

4

Field studies of immunisation of 3-4 months old lambs with drug-abbreviated infections

51%

Hadas E., Stankiewicz M.

Acta Parasitologica

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1998

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tom 43

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nr 1

We investigated the ability of young lambs to develop protective immunity to gastro-intestinal nematode following immunisation with drug-abbreviated infection. Thirty, 3-4 month old lambs were randomly allocated to five groups. Groups 1, 2 and 3 were immunised by three Oxfendazole-abbreviated artificial infections of Trichostrongylus colubriformis and Ostertagia circumcincta. Group 1 received three high immunising larval doses. Groups 2 and 3 were immunised in the same way as Group 1 but with 50 and 75% of the larval dose, respectively. Group 4 was treated with Oxfendazole at the same time as Groups 1, 2 and 3, while Group 5 was an untreated control. All groups grazed the same pasture. At the end of experiment the lambs from Groups 1 and 4 had higher liveweight gains and a higher dag weights than Group 5. Mean total faecal worm egg count was reduced in animals from Groups 1-4, compared controls. However, because of the limited efficacy of vaccination of 3-4 month old lambs by drug-abbreviated infections we suggest that immunisation of young lambs may not be practical unless accompanied by appropriate immunostimulation of the gut mucosa.

Ograniczanie wyników

3 Acta Parasitologica

1 Wiadomości Parazytologiczne

2 Hadas E.

2 Stankiewicz M.

2 Wedrychowicz H.

1 Holmes P. H.

1 Tait A.

2 1998

2 1994

Ostertagia circumcincta: surface and cuticular proteins and glycoproteins isolated by biotin-streptavidin affinity chromatography

Surface antigens of exsheathed infective larvae of Ostertagia circumcincta

The effect of anthelminthic treatment or immunisation of ewes during pregnancy on periparturient rise in ewes and liveweight gains of lambs

Field studies of immunisation of 3-4 months old lambs with drug-abbreviated infections