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Several parasitic plants are known to have acquired mitochondrial genes via a horizontal transfer from their hosts. However, mitochondrial gene transfer in this direction has not yet been found in the parasite-rich family Orobanchaceae. Based on a phylogenetic analysis of the mitochondrial atp6 gene in selected species of Orobanche s.l., we provide evidence of a host-to-parasite transfer of this gene in O. coerulescens, which is a Eurasiatic species that parasitises Artemisia (Asteraceae). We did not find the original Orobanche atp6 gene in this species, which suggests that it has been replaced by a gene that was acquired from Asteraceae. In addition, our data suggest the occurrence of a second HGT event in the atp6 sequence – from Asteraceae to Phelipanche. Our results support the view that the transfer of genetic material from hosts to parasites influences the mitochondrial genome evolution in the latter.
Broomrapes (Orobanche spp.) are important parasitic weeds of peas, faba bean and tomatoes and other winter crops in Egypt. They are widespread and are major factors limiting production of these crops. From an extensive survey of Egyptian soils naturally infested with broomrapes, 42 isolates of fungi belonging to genera of Alternaria, Fusarium and Trichoderma were identified as pathogens of broomrapes under laboratory and greenhouse conditions. Three isolates of Trichoderma spp. including T. harzianum T1, T. harzianum T3 and T. viride T2 were further tested for control of Orobanche spp. in peas, faba bean and tomatoes under field conditions. Results of field studies showed that soil treatment with these three fungal agents alone or soil treatment with fungal agents plus aerial spray of glyphosate (50 ppm) was effective in reducing infection of broomrapes and increasing yields of peas, faba bean and tomatoes. The prospect of developing T. harzianum T1, T. harzianum T3 and T. viride T2 as mycoherbicides for control of bromerapes of peas, faba bean and tomatoes in Egypt is discussed in this paper.
The distribution of the genus Orobanche in SE Poland is presented. The study area stretches between the Vistula and the Bug rivers, and comprises the Polish areas of the Lublin-Lwów Upland, theWołyń Upland and the southern part of Polesie. Eight species of the genus Orobanche: O. alba, O. alsatica, O. arenaria, O. caryophyllacea, O. elatior, O. lutea, O. pallidiflora, O. picridis, were collected during floristic investigations conducted between 1999 and 2010. The hosts, abundance and habitat preferences at the localities are given and a supplemented map of the distribution in SE Poland is included.
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