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Genetic relatedness among basil accessions including six species of Ocimum (O. basilicum L., O. americanum L., O. x citriodorum L., O. minimum L., O. gratissiumum L., O. tenuiflorum L.) and six botanical varieties or cultivars of O. basilicum L. [var. basilicum L. cv. Genovese, var. basilicum L. cv. Sweet Basil, var. difforme Benth., var. purpurascens Benth., cv. Dark Opal, and var. thyrsiflorum (L.) Benth.] were analyzed by random amplified polymorphic DNA (RAPD) markers. Five primers were used for amplification. They yielded a total of 102 easily scorable polymorphic markers. Jaccard indices were calculated and phylogenetic relationships were determined by neighbor-joining cluster analysis. Different Ocimum species were clearly divided into separate clusters with the exception of O. minimum accessions, which clustered together with O. basilicum accessions. In addition to morphological, chemical and crossability data, RAPD analysis can be a useful tool for resolving existing problems in identification and classification of basils.
One of the most popular and usable Aromatic plants are kinds of Basilica (Ocimum sp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must demonstrate this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on three ecotypes of Basilica seed germination. The base of this experiment was a randomized block design with three replications. We used 50 seeds in Falcon tube (value=15ml) and recorded the weight and value of seeds in tube. Then we added 5ml of water in tube and shacked after moisturized and control and recorded the Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Thus, the best suggestion for the produce of mucilage from the basil seeds, the best time is the first quadrature of the moon.
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