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The changes in actin content and polimerization during hepatoma Morris 5123 growth process. The effect of methotrexate

100%

Otrocka M., Nowak D., Malicka-Blaszkiewicz M.

Folia Histochemica et Cytobiologica. Supplement

1997

tom 35

nr 2

Identification of actin from hepatoma Morris 5123 cells

80%

Nowak D., Kochman A., Malicka-Blaszkiewicz M.

Acta Biochimica Polonica

1999

tom 46

nr 4

The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118; Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (Mr 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Folia Histochemica et Cytobiologica. Supplement

2 Malicka-Blaszkiewicz M.

2 Nowak D.

1 Kochman A.

1 Otrocka M.

1 1999

1 1997

Wyniki wyszukiwania

The changes in actin content and polimerization during hepatoma Morris 5123 growth process. The effect of methotrexate

Identification of actin from hepatoma Morris 5123 cells