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Effects of elevated temperature and soil pollution with fluorine on host-pathogen relations were studied in seedlings of the pedunculate oak (Quercus robur L.) inoculated with oak powdery mildew (Microsphaera alphitoides Griff. et Maubl.) and control seedlings. The plants were grown for 1month in elevated temperature (on average by 1.6°C) and soil pollution with sodium fluoride (330 ppm F). The above factors did not have any significant effect on nitrogen content of leaves or on concentrations of metabolites favourable to growth and development of the fungal pathogen (total non-structural carbohydrates, including soluble carbohydrates and starch) and those unfavourable to fungi (soluble phenols, condensed tannins and lignins). The elevated temperature and fluorine pollution did not affect the leaf infection rate. However, a significant temperature × pollution interaction was observed in inoculated seedlings. At the elevated temperature, fluorine caused a less severe infection by powdery mildew. This could be due to a direct toxic effect of fluorine on the pathogen or by an indirect influence, resulting from changes in levels of other metabolites, which were not analysed in this study. The inoculation of oak seedlings with powdery mildew caused a decline in the carbohydrate content of leaves but did not have any significant effect on levels of other analysed metabolites. However, it significantly affected the distribution of phenols and lignins in oak leaves. Those compounds accumulated within necrotic lesions and in adjacent cells. Our results do not enable drawing definite conclusions on effects of a slight rise in temperature and a relatively low level of fluorine pollution of the soil on relations between the pedunculate oak and oak powdery mildew. Lower values of the leaf infection rate in seedlings growing in elevated temperature and fluorine pollution suggest that in warmer years a lower level of infection by M. alphitoides may be expected in areas affected by fluorine pollution.
The aim of this study was to verify descriptions of the morphology of the fungus responsible for powdery mildew on oak leaves of nursery seedlings and of young trees in forest stands. Infected, fully developed leaves from apical parts of stems of Quercus robur or Q. petraea were collected in two national parks and in six forest districts in Poland. The taxonomic characteres of all specimens were generally consistent with those given in keys for Microsphaera alphitoides. However, the limoniform shape of conidia, characteristic of two sites (nurseries), has not been found in available synopses. Limoniform conidia were significantly longer and wider than others. Agreat variety of forms of mycelium on leaves were observed. The mycelium appeared in three forms: (1) floccose or felted oval patches (always with yellow or brown discoloration), (2) faveolate oval patches (often with yellow or brown discoloration), and (3) felted or mealy mycelium along veins (rarely with discoloration). Mycelium without limoniform conidia appeared in all forms, while mycelium with limoniform conidia appeared only in the form of patches, always causing leaf discoloration.
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