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1

Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018

100%
Kozdrun W., Stys-Fijol N., Czekaj H., Piekarska K., Niczyporuk J. S., Stolarek A.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 4
2
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Polymerase chain reaction for the differentiation of Marek's disease virus strains

84%
Kozdrun W., Samorek-Salamonowicz E., Czekaj H.
Bulletin of the Veterinary Institute in Puławy
|
2001
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tom 45
|
nr 1
The aim of this study was an attempt to apply PCR for diagnosis of Marek's disease. Eighteen field Marek's disease virus strains and 4 standard strains: virulent HPRS₁₆ strain and attenuated CVI 988 strain (serotype 1 - MDV-1), apathogenic SB 1strain (serotype 2 - MDV-2) and non - pathogenic turkeys HVT FC 126 strain (serotype 3 MDV-3) were used. Chicken anaemia virus (CAV) and infectious laryngotracheitis virus (ILTV) were used as control of PCR. The virus strains were cultured in CEF and total DNA was isolated by A@A Biotechnology kit. Three pairs of primers were used: for gene A of serotype 1, for 132 bp sequence of serotype 1 and for gene A of serotype 3. PCR was carried out under the following conditions: 94°C - 1 min (denaturation), 55°C - 30 s (annealing), 72°C -30 s (elongation). PCR products were analysed by electrophoresis in 2% agarose gel at 100 V/h. The PCR product of 314 bp was obtained from all field strains and HPRS₁₆ standard after the primers for gene A of serotype 1 were used. After the primers for 132 bp sequence of serotype 1 were used, 434 bp fragment for 19 DNA samples derivied from field strains and standard HPRS₁₆ strain and 1033 bp fragment from attenuated CVI 988 strain were obtained. Application of primers for gene A serotype 3 allowed the detection of 436 bp product only from non-pathogenic HVT FC 126 strain (serotype 3). These results indicated that the PCR technique can be used for the differentiation of MDV strains.
3

Duplex PCR assay for detection and differentiation of pathogenic and vaccine strains of MDV serotype 1

84%
Krol K., Samorek-Salamonowicz E., Kozdrun W., Wozniakowski G.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 3
To increase the diagnostic capacity of PCR, duplex PCR for the detection of the Meq gene and 132 bp sequence of Marek's disease virus (MDV), serotype 1 has been developed. The reaction enabled the differentiation of vaccine and field strains of MDV among serotype 1 of the virus. Additionally, it gave preliminary information about eventual vaccination with the Rispens strain.
4

Sequence analysis of meq oncogene among Polish strains of Marek's disease

67%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 2
5

Occurrence of main Marek's disease genes during infection of chickens

67%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 2
123-127
The aim of this study was the determination of the occurence of ICP4, pp38, meq, and LAT genes of Marek's disease virus (MDV) and its rate of replication in internal organs of infected chickens. The genes were determined using PCR, while the virus replication was measured by real-time PCR method. The results have shown the presence of ICP4 and pp38 genes starting from the 3rd d p.i. while meq oncogene was detected from the 7 d p.i. During the whole period of the experiment, no presence of latency associated transcript gene (LAT) was found. The virus replicated most intensively in the spleen and bursa of Fabricius then in the thymus, liver, and lungs. The results imply a role of the main genes ICP4 and pp38 in early cytolytic infection of chickens, and the further occurrence of meq oncogene associated with tumourgenesis. The highest dynamics of MDV replication in the lymphoid organs - bursa of Fabricius, thymus, and spleen indicates its stringent association with lymphoid cells and tissues.
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