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1

Taksonomia rodzaju Lyssavirus

100%
Marzec A., Smreczak M., Zmudzinski J. F.
Medycyna Weterynaryjna
|
2016
|
tom 72
|
nr 05
The article presents the current state of knowledge on the taxonomy of the genus Lyssavirus. Viruses belonging to Rhabdoviridae family cause acute encephalitis (inflammation of the brain) which almost irrevocably results in death. The best known representative of this genus is the rabies virus, which until the 1950s was regarded as unique. The use of serological and molecular methods allowed for the discovery a number of new lyssaviruses and the introduction of new terminology regarding the genus Lyssavirus on the basis of various criteria proposed by ICTV was needed. Currently, the Lyssavirus genus includes 14 species, but taking into account the number of new species discovered in recent years it can be hypothesized that such variations may increase in the future, adding new members.
2

Evaluation of direct metagenomics and target enriched approaches for high-throughput sequencing of field rabies viruses

88%
Orlowska A., Iwan E., Smreczak M., Rola J.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 4
3

Zwalczanie wirusowej posocznicy krwotocznej [VHS]

51%
Olesen N. J.
Magazyn Przemysłu Rybnego
|
2000
|
nr 2
44-46
4

Comparison of real-time PCR and heminested RT-PCR methods in the detection of rabies virus infection in bats and terrestrial animals

51%
Orlowska A., Smreczak M., Trebas P., Zmudzinski J. F.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
|
nr 3
The aim of the paper was to compare the real-time PCR with the heminested RT-PCR method, both applied for the detection of nucleoprotein gene of rabies viruses in bats and terrestrial animals. The study involved 32 rabies virus isolates collected from bats and terrestrial animals coming from different regions of Poland. For both methods, the sensitivities related to TCID₅₀/mL of CVS virus were estimated. The comparison of the methods revealed that the TaqMan PCR was 10-fold more sensitive than the heminested RT-PCR and the detection of rabies virus by this method was possible from 0.1 TCID₅₀/mL on up. The use of the heminested RT-PCR allowed for detection of rabies virus from 1 TCID₅₀/mL on up. Next, the examination of 32 archive samples using both methods revealed 23 positive samples and nine negative samples. The agreement between the results obtained by the methods was 100%. It confirms using the real-time PCR and heminested RT-PCR in laboratory diagnosis of rabies in terrestrial animals and bats. However, the real-time PCR does not require visualisation of the amplification product in agarose gel and the results are observed during the run of the test, which makes the method more rapid than the heminested RT-PCR. Additionally, it is done in a single closed tube and does not require multiple transfer of materials like at the heminested RT-PCR, thus making the virus detection faster and minimising the opportunity for cross-contamination.
5

Application of heminested RT-PCR to the detection of EBLV1 and classical rabies virus infections in bats and terrestrial animals

51%
Smreczak M., Orlowska A., Trebas P., Zmudzinski J. F.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 1
The paper describes application and optimisation of hnRT-PCR in the detection of the fragment of nucleoprotein gene of Lyssaviruses (genotypes 1 and 5) as a method for laboratory diagnosis of rabies in terrestrial animals and bats. The heminested RT-PCR, because of its sensitivity and rapidity, it may be used as a technique for rabies diagnosis. The method can be applied both in living animals as well as in the case of post mortem collected samples. It provides not only rapid detection of rabies virus but also gives the material for sequencing of the PCR products for final identification of origin of the strain and epidemiological analysis.
6
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Post-exposure anti-rabies prophylaxis in Lublin Province [Eastern Poland] in 2004-2005

51%
Tomasiewicz K., Fota-Markowska H., Krzowska-Firych J., Krawczuk G.
Annals of Agricultural and Environmental Medicine
|
2006
|
tom 13
|
nr 2
Rabies is considered a disease of the highest mortality rate and all humans are vulnerable to infection. Specific anti-rabies immunoprophylaxis is the only efficient method of protection. The analysis of indications for active alone and active and passive immunization among patients reported to the dispensary of rabies prophylaxis in the Department of Infectious Diseases of Medical University of Lublin (eastern Poland) in 2004-2005 is presented. Prophylactic procedures were applied in 120 persons (14.98% of overall number consulted). Passive immunization, i.e. rabies immune globulin, was administered in 1 person (0.12%). In 2004, 64 persons (7.99%) received active vaccination, and 56 patients (6.99%) were vaccinated in 2005. Most of vaccinated patients lived in an urban area where the risk of rabies should be lower; however, in cities like Lublin there is a higher risk of being bitten by homeless animals. The most common species with rabies suspicion were dogs and cats. The decrease in the number of patients bitten by animals with confirmed rabies in Lublin province, and of the number of cases of animal rabies may indicate that oral vaccination of red foxes, representing a main reservoir of rabies virus in Poland, has been shown to be effective.
7

Lyssavirus spp. – wirusy wścieklizny jako wciąż aktualny problem

45%
Galazka P., Kaczor P., Grzelakowska K., Leis K.
Postępy Mikrobiologii
|
2019
|
tom 58
|
nr 2
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