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Essential oil of Artemisia annua L. was investigated to find out its toxicity and physiological aspects on the slug Agriolimax agrestis, in controlled conditions (8±1°C, 75±5 RH and 14:10 LD). The slugs received different concentrations of essential oil treated radish leaves in methanol, while the control received methanol alone. LC10, LC30, LC50 and LC90 values were estimated at 4.67, 5.3, 5.81, 7.25%, respectively. The effect of the essential oil on some important enzymatic components like; cytochrome P450 monnooxygenase, acid phosphatase, alkaline phosphatase, lipase, amylase and protease were significantly increased compared to the control. These results indicate that the plant Artemisia annua L. not only shows toxicity but also shows some irreversible effect on some important biochemical components and deserves further investigation.
All previously known former Yugoslavian terrestrial slugs of the families Arionidae, Milacidae, Limacidae and Agriolimacidae have been discussed in this paper. The author conducted his research in the former Yugoslavia's territory in 1982-1983. He has also examined material from museums and private collections. The taxa, from the family to the species, are provided with full synonymy, more important references, descriptions of the external appearance and systematically useful anatomical characters, information on ecology and distribution in the world, list of new and already known localities, and comments, which - e.g. - help to distinguish a particular taxon from other ones. There are also keys to all the families and species, characteristics of the former Yugoslavia's slug fauna figures of all the species (nearly all made by the author himself) and distribution maps on a simplified UTM grid. The occurrence of 48 species has been established. Six other, in the author's opinion, were erroneously recorded in literature and should not be reckoned among the fauna of this area. Besides, 11 species mentioned by former authors still have a completely unclear status. Two species new to science have been described, i.e. Tandonia rara and Deroceras maasseni, and 20 names synonymized.
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