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Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
The research material consisted of tulip plants of ‘Leen van der Mark’ cv. The effect of two preparations was evaluated: Sunspray 850 EC and Sunspray Ultra-Fine, in which the active substance is refined paraffinbased mineral oil. Preparations were applied at three concentrations: 1, 1.5, and 2%. Research plots were sprayed three times every 7 days. The first treatment was performed at the turn of the second and third decade of April. Starting from the beginning of May, observations of plant colonization by aphids were conducted every 14 days. The observations were terminated at the end of June. It was found that spraying using mineral oils Sunspray 850 EC and Sunspray Ultra-Fine of tulip ‘Leen van der Mark’ significantly affect the degree of virus infection and infection due to disease-causing pathogens in relation to the untreated plants. Plants sprayed with mineral oil at the lowest concentration (1%) were in better health condition and were less attractive to aphids. Increasing the concentration of oil and the number of treatments intensified the phytotoxic reaction of tulips manifested as chlorosis with varying intensity. They were not, however, apparent effect on plant growth and development. The chlorophyll content (expressed as greenness index) in the tulip leaves treated with mineral oil was lower as compared to untreated leaves, when plants were sprayed three times using Sunspray Ultra-Fine oil at a concentration of 2%.
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