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1

Comparative analysis of the gene encoding VP60 capsid protein in various strains of the RHD (rabbit haemorrhagic disease) virus

100%
Hukowska-Szematowicz B., Manelska A., Tokarz-Deptula B., Deptula W.
Advances in Agricultural Sciences
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2011
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tom 14
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nr 1-2
2
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Characteristics of a new variant of rabbit haemorrhagic disease virus – RHDV2

100%
Bebnowska D., Niedzwiedzka-Rystwej P.
Acta Biologica
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2019
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tom 26
3

Phylogenetic analysis of partial capsid protein gene of rabbit haemorrhagic disease virus [RHDV] strains isolated between 1993 and 2005 in Poland

100%
Chrobocinska M., Mizak B.
Bulletin of the Veterinary Institute in Puławy
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2007
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tom 51
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nr 2
The 18 RHDV strains, isolated between 1993 and 2005 in Poland, as well as one standard strain were used in these studies. The RT-PCR technique was carried out in order to amplify the 538 bp fragment located in the variable region of gene encoding capsid protein (VP60). The phylogenetic analysis of nucleotide sequences of 18 Polish and 25 foreign strains revealed that Polish strains were clustered to 4 genogroups. One of them from 1993 was grouped together with old European strains isolated between 1987 and 1990. Thirteen strains isolated between 1994 and 2004, as well as two strains isolated in 1994 and 1996, were clustered to two separate genetic groups. Two strains detected in 2004 and 2005, were located in genogroup of antigenic variants. The maximum nucleotide divergence among Polish strains (10.3%) was found between strain isolated in 1996 and antigenic variants. The alignment of deduced amino acid sequences of the VP60 confirmed the localisation of 2 Polish strains in genetic group of antigenic variants. The identical amino acid substitutions in the same positions, like in other antigenic variants, were observed. The relation between antigenic properties and genetic variations of strains was taken under consideration.
4

Analysis of the fragment of capsid protein gene sequences of Polish strains of European brown hare syndrome virus

100%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
|
2002
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tom 46
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nr 2
5

Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus

100%
Gromadzka B., Szewczyk B., Konopa G., Fitzner A., Kesy A.
Acta Biochimica Polonica
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2006
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tom 53
|
nr 2
Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.
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