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Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing

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Zaczek A., Brzostek A., Gorna A., Sajduda A., Wojtasik A., Dziadek J.

Polish Journal of Microbiology

2013

tom 62

nr 1

The current “gold standard” in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discriminatory power of FLiP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative to the other typing methods.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Brzostek A.

1 Dziadek J.

1 Gorna A.

1 Sajduda A.

1 Wojtasik A.

1 Zaczek A.

1 2013

Wyniki wyszukiwania

Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing