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Production and evaluation of doubled haploid wheat lines

100%

Pauk J., Kertesz Z.

Journal of Applied Genetics

1997

tom 38

nr 4

F₁, F₂ populations as well as F₃ and F₄ wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.

Genetic mapping and tagging of wheat genes using RAPD, STS and SSR markers

100%

Khlestkina E. K., Pestsova E. G., Salina E., Roder M. S., Arbuzova V. S., Koval S. F., Borner A.

Cellular and Molecular Biology Letters

2002

tom 07

nr 2B

We applied SSR markers for mapping genes determining red coleoptile colour in wheat (Rc1, Rc2, Rc3) using F2 populations. All three genes map at about 15 to 20 cM distally from the centromere of chromosomes 7AS, 7BS and 7DS, respectively. The locations of the glume colour (Bg, Rg1) and glume hairiness (Hg) genes relative to the SSR markers of the homoeologous chromosomes group 1 were determined using molecular analysis of near-isogenic lines (NILs). One RAPD marker for the vernalisation response gene Vrn-A1 was identified by screening 95 random primers against two pairs of NILs. New PCR (STS) markers were developed based on RFLP-markers PSR426 (5A, 5B, 5D) and PSR1201 (1A, 5A, 5B). Analysis of nulli-tetrasomic and near-isogenic lines of wheat using the STS markers developed gave an indication that these new STS markers have the same chromosomal and intrachromosomal positions as the correspondent RFLP markers. Therefore, they could be used for mapping and/or tagging the vernalisation response (Vrn-A1, Vrn-B1, Vrn-D1) and homoeologous pairing (Ph1) genes.

Linkages in Pisum L. VII. Locus for the sterile gene calf [cabbage leaf]

100%

Swiecicki W. K., Wolko B., Kruszka K.

Journal of Applied Genetics

1998

tom 39

nr 2

Genetical analyses were conducted to find linkages and the locus of the gene calf on the Pisum chromosome map. The recessive, pleiotropic gene calf (enlarged and undulated leaflets, stipules, flowers and pods, plant sterile), artificially induced (the initial line-Large Podded G-20, the mutagene-DES and NMU) was described by Sharma in 1975. An identical mutant gene at the same locus was isolated in our research (the initial line - cv. Pegro, the mutagene - fast neutrons). Two lines were included in the Pisum gene bank - the type line for the gene calf - Wt 15873 and the representative line - Wt 16024. In linkage studies the representative line was crossed with tester lines bearing gene markers. Analyses of dihybrid segregation in F₂ generations revealed linkages of the gene calf with chromosome 2 markers. Two isozymic markers helped to reveal the calf locus on chromosome 2 with the following gene order: Orp - Calf - K - Pgm-p - Fum. This is in agreement with the current Pisum linkage map.

Ograniczanie wyników

2 Journal of Applied Genetics

1 Cellular and Molecular Biology Letters

1 Arbuzova V. S.

1 Borner A.

1 Kertesz Z.

1 Khlestkina E. K.

1 Koval S. F.

1 Kruszka K.

1 Pauk J.

1 Pestsova E. G.

1 Roder M. S.

1 Salina E.

1 Swiecicki W. K.

1 Wolko B.

1 2002

1 1998

1 1997

Wyniki wyszukiwania

Production and evaluation of doubled haploid wheat lines

Genetic mapping and tagging of wheat genes using RAPD, STS and SSR markers

Linkages in Pisum L. VII. Locus for the sterile gene calf [cabbage leaf]