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PCo-A analysis of strawberry germplasm used in European breeding programs, based on evaluation of DNA polymorphism of investigated plants

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Kuras A., Korbin M.

Journal of Fruit and Ornamental Plant Research

2010

tom 18

nr 2

7-16

The level of genetic relatedness of ninety-six strawberry cultivars, released in different breeding centres of seventeen countries, was estimated based on analysis of their DNA polymorphism. Five hundred fifty-eight polymorphic amplicons, with a size range from 80 to 2600 bp, were generated in PCRs carried out on the template of DNA isolated from plants representing all analyzed cultivars. In RAPD reactions, polymorphic bands covered 58% of the total number of PCR products, while in ISSR, SSR and selective AFLP, the polymorphic DNA fragments covered 75%, 70% and 67% of all amplicons, respectively. Data concerning DNA polymorphism were assembled using the PCo-A method (Principal Component Analysis), and then referred to information about country of origin and pedigree described by the breeders. The results showed that contemporary breeding uses genetic resources in a very narrow range. Consequently, the cultivars released in individual breeding centres presented a very close relationship and were grouped in one, or at most two, genetic clusters.

Ograniczanie wyników

1 Journal of Fruit and Ornamental Plant Research

1 Korbin M.

1 Kuras A.

1 2010

Wyniki wyszukiwania

PCo-A analysis of strawberry germplasm used in European breeding programs, based on evaluation of DNA polymorphism of investigated plants