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1

Application of real-time reverse transcription polymerase chain reaction for the detection of SVDV

100%
Niedbalski W.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
Application of real-time RT-PCR (rRT-PCR) for detection of swine vesicular disease virus (SVDV) in samples of archival SVDV isolates and clinical samples collected from SVDV infected pigs was described. A primer set that targets the IRES region of the SVDV genome and TaqMan probe specific for a highly conserved region in SVDV RNA IRES region were used. The assay detected viral RNA in all tested archival strains of SVDV isolated in Europe during years 1972-73 and 1992 as well as in clinical samples collected from experimentally infected pigs. The rRT-PCR can provide quantitative and qualitative information and is more sensitive and faster to perform than the conventional RT-PCR.
2
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Phylogenetic analysis of Polish isolates of swine vesicular disease virus [SVDV]

100%
Niedbalski W.
Bulletin of the Veterinary Institute in Puławy
|
1999
|
tom 43
|
nr 2
The aim of this studies was the genetic analysis of Polish SVDV isolates from 1972-73. The direct nucleotide sequencing technique of PCR products from the 1D (VP1) structural polipeptyde coding region was applied. The genetic differences between Polish isolates and other European and Japanese ones from 1966-1994 were determined by comparing their nucleotide sequences. No considerable genetic divergences were found between Polish isolates and the others in 1972-1981 . This suggests a close relation and common origin of all isolates belonging to genogroup II. The considerable genetic differences (10-15%) were found between Polish strains from 1972-73 and West European isolates from 1991/92 (genogroup III) and 1993/94 (genogroup IV). These results indicated that distribution of isolates within these genogroups appears to be more related to the year of their collection than to their geographical origin. The obtained results can be important information for epidemiological studies of SVD in Europe.
3
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Genetic analysis of Polish isolate of swine vesicular disease virus [SVDV POL-73] by RT-PCR and nucleotide sequencing of the VP1-2A coding region

80%
Niedbalski W.
Bulletin of the Veterinary Institute in Puławy
|
1998
|
tom 42
|
nr 1
A nucleotide sequence analysis by RT-PCR and cycle sequencing of the Polish isolate of swine vesicular disease virus (SVDV) was performed. The RT-PCR products pattern and VP1/2A coding region sequence were compared to the other available European isolates of SVDV and the human Coxackie virus isolate CAV-16. The results of the RT-PCR show that it is possible to detect different isolates of the SVDV however Coxackie virus-specific PCR products were obtained only by the use of one from three selected primer pairs. A sensitivity of the used RT-PCR was equivalent to that of virus isolation in cell culture and even higher than the ELISA method. The considerable nucleotide similarity between the Polish isolate UKG27/72 and the Japan 76 SVDV isolates (99% and 98%, respectively) as well as the CAV-16 (96%) was found. SVDV Poland 73 was genetically less related (about 88%) to the Dutch and Italian isolates from the recent outbreaks of disease in 1992. Presented results confirmed the quasispecies character of these picornaviruses.
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