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Species identification of Diplostomum pseudospathaceum Niewiadomska, 1984 and D.paracaudum [Iles, 1959] metacercariae using DNA polymorphism amplified by arbitrary primers

100%

Laskowski Z.

Acta Parasitologica

1996

tom 41

nr 1

Metacercariae of Diplostomum pseudospathaceum and D. paracaudum were examined for genetic differences by random amplified polymorphic DNA analysis. Eleven different oligonucleotide decamers with arbitrary DNA sequence were tested as primers to amplify DNA from D. pseudospathaceum and D. paracaudum. Depending on the primer, the number of reproducible amplified fragments varied from 7 to 15, ranging in size from 0.2 to 2.0 kb DNA. Reproducible differences in patterns allowed differentiation of the two species with each primer. RAPD patterns unambiguously discriminated between D. pseudospathaceum and D. paracaudum, allowing good species identification.

Distribution of serotonin immunoreactive [5HI-IR] neurons and fibres in metacercaria of Diplostomum paracaudum [Iles, 1959] [Digenea]

75%

Niewiadomska K., Maslinska D., Czubaj A., Pomorski P.

Acta Parasitologica

1993

tom 38

nr 1

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Species identification and phylogenetic analysis of six species of Diplostomum Nord. [Digenea]

51%

Niewiadomska K., Borsuk P., Laskowski Z.

Wiadomości Parazytologiczne

1998

tom 44

nr 3

Ograniczanie wyników

2 Acta Parasitologica

1 Wiadomości Parazytologiczne

2 Laskowski Z.

2 Niewiadomska K.

1 Borsuk P.

1 Czubaj A.

1 Maslinska D.

1 Pomorski P.

1 1998

1 1996

1 1993

Wyniki wyszukiwania

Species identification of Diplostomum pseudospathaceum Niewiadomska, 1984 and D.paracaudum [Iles, 1959] metacercariae using DNA polymorphism amplified by arbitrary primers

Distribution of serotonin immunoreactive [5HI-IR] neurons and fibres in metacercaria of Diplostomum paracaudum [Iles, 1959] [Digenea]

Species identification and phylogenetic analysis of six species of Diplostomum Nord. [Digenea]