Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: Deinococcus radiodurans

Sortuj według:

Ogranicz wyniki do:

Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli

100%

Filipkowski P., Pietrow O., Panek A., Synowiecki J.

Acta Biochimica Polonica

2012

tom 59

nr 3

A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinant trehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography and characterized. The expressed enzyme is a homodimer with molecular mass of 126.9 kDa and exhibits the highest activity of 11.35 U/mg at pH 7.6 and at 30°C. DraTreS activity was almost unchanged after 2 h preincubation at 45°C and pH 7.6, and retained about 56% of maximal value after 8 h incubation at 50°C. The DraTreS was strongly inhibited by Cu2+, Hg2+, Zn2+, Al3+ and 10 mM Tris. The Km value of maltose conversion was 290.7 mM.

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

A new division of bacterial UvrA homologues

84%

Marszalkowska M., Bil M., Kreft L., Olszewski M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 1

Structural basis for the antibacterial activity of the 12-membered-ring mono-sugar macrolide methymycin

67%

Auerbach T., Mermershtain I., Bashan A., Davidovich C., Rozenberg H., Sherman D. H., Yonath A.

Biotechnologia

2009

nr 1

24-35

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Biotechnologia

1 Auerbach T.

1 Bashan A.

1 Bil M.

1 Davidovich C.

1 Filipkowski P.

1 Kreft L.

1 Marszalkowska M.

1 Mermershtain I.

1 Olszewski M.

1 Panek A.

1 Pietrow O.

1 Rozenberg H.

1 Sherman D. H.

1 Synowiecki J.

1 Yonath A.

1 2013

1 2012

1 2009

Wyniki wyszukiwania

Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli

A new division of bacterial UvrA homologues

Structural basis for the antibacterial activity of the 12-membered-ring mono-sugar macrolide methymycin