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Dynamika wzrostu i plonowanie bielunia indianskiego [Datura innoxia Mill.] na tle warunkow pogodowych

100%

Pakula I., Buczkowska H., Bednarek H.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EEE: Horticultura

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2006

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tom 16

93-100

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Wpływ sposobu uprawy na plonowanie bielunia indiańskiego (Datura innoxia Mill.) oraz zawartość alkaloidów w zielu

67%

Pakula I., Buczkowska H.

Folia Horticulturae. Suplement

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2006

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nr 2

3

Rosliny narkotyczne i halucynogenne w zatruciach mlodziezy. Cz.III

67%

Lamer-Zarawska E.

Wiadomości Zielarskie

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2001

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tom 43

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nr 12

14-16

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A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures

67%

Dechaux C., Boitel-Conti M.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated the alkaloid pathway, modified gene expression, and cultivated and optimized hairy root growth in a bioreactor. Datura innoxia whole plant was transformed by Agrobacterium rhizogenes, and the obtained hairy roots were studied for their tropane alkaloid production. Optimization of medium led to an increase of scopolamine production in hairy root culture of Datura innoxia by increasing the produced biomass, but genetic engineering seems to be the best way to increase the accumulation of scopolamine. These root cultures were not able to stably overaccumulate scopolamine. The involvement of putative enzymatic regulation of the tropane alkaloid pathway is discussed.

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Yield of a herb [Datura innoxia Mill.] and content of alkaloids depending on development stage of harvested plants

67%

Buczkowska H., Pakula I.

Herba Polonica

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2007

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tom 53

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nr 3

The experiments were carried out in 2004-2006 in the Department of Vegetable and Medicinal Plants, Agricultural University in Lublin. The aim of presented work was to evaluate a development stage in which should the Datura innoxia plants be harvested to obtain herb with high content of alkaloids per area unit. Plants were harvested: 1 - in a stage of vegetative growth, 2 - in the beginning of flowering, 3 - in the beginning of fructification. The effects of weather conditions on air-dry herb yield (17.0-23.2 kg-100 m~2) and content of tropane alkaloids (0.166-0.224%) was stated. Significantly lower mean herb yield was obtained from plants harvested in vegetative growth stage. Significant relationship between tropane alkaloids content and development stage of plants was not stated. Quantity of tropane alkaloids obtained in cultivation of Datura innoxia (22.32-55.57 gl 00 nr2) depended more on high value of air-dry herb yield per area unit (12.9-28.5 kg-100 m2) than on content of these active substances in material (0.150-0.236%).

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Nasiona bielunia dziedzierzawy przyczyna niebezpiecznych zatruc

59%

Szypula W.

Wiadomości Zielarskie

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2001

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tom 43

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nr 10

14-15

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Androgenic development: a fascinating embryo formation process

51%

Goralski G., Matthys-Rochon E., Vergne P., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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1999

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tom 41

The subject of this review is androgenesis as a process of embryo development from microspores and pollen. A number of factors govern androgenesis, but genotype, the stage of microspore development and in vitro culture conditions are the most important. We summarize some recent work employing a variety of experimental approaches to study this fascinating phenomenon of embryo formation.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

2 Wiadomości Zielarskie

1 Annales Universitatis Mariae Curie-Skłodowska. Sectio EEE: Horticultura

1 Folia Horticulturae. Suplement

1 Herba Polonica

3 Buczkowska H.

3 Pakula I.

1 Bednarek H.

1 Boitel-Conti M.

1 Dechaux C.

1 Goralski G.

1 Lamer-Zarawska E.

1 Matthys-Rochon E.

1 Przywara L.

1 Szypula W.

1 Vergne P.

1 2007

2 2006

1 2005

2 2001