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1

Effect of null mutations in dnaK and dnaJ genes on conjugational DNA transfer, proteolysis and novobiocin susceptibility of Escherichia coli

100%
Modrzewska M., Karpinski P., Grudniak A., Wolska K. I.
Acta Microbiologica Polonica
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2002
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tom 51
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nr 3
2

Horizontal DNA transfer between bacteria in the environment

100%
Wolska K. I.
Acta Microbiologica Polonica
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2003
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tom 52
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nr 3
3

Odyssey of Agrobacterium T-DNA

100%
Ziemienowicz A.
Acta Biochimica Polonica
|
2001
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tom 48
|
nr 3
Agrobacterium tumefaciens, a plant pathogen, is characterized by the unique feature of interkingdom DNA transfer. This soil bacterium is able to transfer a fragment of its DNA, called T-DNA (transferred DNA), to the plant cell where T-DNA is integrated into the plant genome leading to "genetic colonization" of the host. The fate of T-DNA, its processing, transfer and integration, resembles the journey of Odysseus, although our hero returns from its long trip in a slightly modified form.
4

Various cationic carriers for in vitro transfection of tumour and endothelial cell lines

100%
Zemlinska B., Sochanik A., Missol-Kolka E., Szala S.
Acta Biochimica Polonica
|
2002
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tom 49
|
nr 1
We compared the efficiency of in vitro DNA transfer into selected tumor and endo­thelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.
5

Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization

84%
Budryk M., Cichon T., Szala S.
Acta Biochimica Polonica
|
2001
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tom 48
|
nr 3
The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.
6
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Plant in vitro cultures as a tool in functional studies of ABC (ATP-binding-cassette) transporters

67%
Jarzyniak K., Biala W., Banasiak J., Jasinski M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
7

Gene insertion into strawberry leaf disk regeneration system using particle bombardment

67%
Sawahel W., Saker M.
Cellular and Molecular Biology Letters
|
1997
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tom 02
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nr 3
Strawberry has previously been transformed using Agrobacterium-mediated DNA transfer. In this paper, we present a process for delivering foreign genes into strawberry leaf disks using particle bombardment. Expression of foreign DNA into strawberry leaf disks (Fragaria X ananassa Duch.) was detected - using in situ GUS assay-following bombardment with tungsten particles coated with plasmid DNA (pBI221.23) that coded for the selectable (hygromycin phosphotransferase [hpt] and the screenable (β-glucuronidase [GUS]) marker genes. Both genes are under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. The criteria of stability of phenotypes after the removal of selective pressure, Southern blot hybridization and segregation analysis were used to confirm the mitotic stability of the foreign gene and its stable integration into the strawberry genome progeny. The relative simplicity of this system recommends its future use for the production of genetically modified strawberry.
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