Wyniki wyszukiwania

1

Mammalian DNA methyltransferases

100%

Siedlecki P., Zielenkiewicz P.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.

2

Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes

100%

Piekarowicz A., Weglenska A.

Acta Microbiologica Polonica

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1994

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tom 43

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nr 2

3

Novel procedure for the detection of 5-methylcytosine

100%

Radlinska M., Skowronek K.

Acta Microbiologica Polonica

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1998

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tom 47

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nr 4

4

Cloning and characterization of M.LmoA118I, a novel DNA: m4C methyltransferase from the Listeria monocytogenes phage A118, a close homolog of M.NgoMXV

100%

Bujnicki J. M., Radlinska M.

Acta Microbiologica Polonica

|

2001

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tom 50

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nr 2

5

Molecular phylogenetics of DNA 5mC-methyltransferases

100%

Bujnicki J. M., Radlinska M.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 1

6

Cloning and preliminary characterization of a GATC-specific beta2-class DNA:m6A methyltransferase encoded by transposon Tn1549 from Enterococcus spp.

84%

Radlinska M., Piekarowicz A., Galimand M., Bujnicki J. M.

Polish Journal of Microbiology

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2005

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tom 54

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nr 3

A recent study revealed a subfamily of N6-adenine (m⁶A) methyltransferases that comprises a few functionally studied eukaryotic members acting on mRNA and prokaryotic members acting on DNA as well as numerous uncharacterized open reading frames. Here, we report cloning and functional characterization of a prokaryotic member of this family encoded by transposon Tn1549 from Enterococcus spp.

7

Site-directed mutagenesis defines the catalytic aspartate in the active site of the atypical DNA: m4C methyltransferase M.NgoMXV

84%

Radlinska M., Bujnicki J. M.

Acta Microbiologica Polonica

|

2001

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tom 50

|

nr 2

8

Role of epigenetic DNA alterations in the pathogenesis of systemic lupus erythematosus

84%

Januchowski R., Prokop J., Jagodzinski P. P.

Journal of Applied Genetics

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2004

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tom 45

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nr 2

9

Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells

67%

Savickiene J., Treigyte G., Borutinskaite V.-V., Navakauskiene R.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 4

DNMT inhibitors are promising new drugs for cancer therapies. In this study, we have observed the antileukemic action of two diverse DNMT inhibitors, the nucleoside agent zebularine and the non-nucleoside agent RG108, in human promyelocytic leukemia (PML) HL-60 cells. Zebularine but not RG108 caused dose- and time-dependent cell growth inhibition and induction of apoptosis. However, co-treatment with either drug at a non-toxic dose and all trans retinoic acid (RA) reinforced differentiation to granulocytes, while 24 or 48 h-pretreatment with zebularine or RG108 followed by RA alone or in the presence of HDAC inhibitors (sodium phenyl butyrate or BML-210) significantly accelerated and enhanced cell maturation to granulocytes. This occurs in parallel with the expression of a surface biomarker, CD11b, and early changes in histone H4 acetylation and histone H3K4me3 methylation. The application of both drugs to HL-60 cells in continuous or sequential fashion decreased DNMT1 expression, and induced E-cadherin promoter demethylation and reactivation at both the mRNA and the protein levels in association with the induction of granulocytic differentiation. The results confirmed the utility of zebularine and RG108 in combinations with RA and HDAC inhibitors to reinforce differentiation effects in promyelocytic leukemia.

10

DNA methylation in states of cell physiology and pathology

67%

Sulewska A., Niklinska W., Kozlowski M., Minarowski L., Naumnik W., Niklinski J., Dabrowska K., Chyczewski L.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 3

11

DNA methyltransferases of Neisseria gonorrhoeae

67%

Piekarowicz A., Radlinska M., Wiernicka-Gnas M.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1996

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tom 44

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nr 3-4

12

Cloning of the dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1

67%

Piekarowicz A., Bujnicki J.

Acta Microbiologica Polonica

|

1999

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tom 48

|

nr 2

13

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Indukowana fenobarbitalem hipermetylacja rejonu promotorowego genu p53 w watrobie szczurow szczepu Wistar

59%

Kostka G., Urbanek-Olejnik K., Wiadrowska B., Bankowski R.

Roczniki Państwowego Zakładu Higieny

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2008

|

tom 59

|

nr 4

455-465

Indukowane niegenotoksycznymi kancerogenami (NGCs) zmiany metylacji DNA rozpatrywane są jako mechanizm ich toksycznego, w tym rakotwórczego działania. Zbadano wpływ fenobarbitalu (PB), na poziom metylacji regionu promotorowego genu p53 w wątrobie szczurów szczepu Wis tar. Zmiany metylacji genu p53 korelowano z syntezą DNA, aktywnością metylotransferaz DNA (DNMTs) oraz z masą wątroby. Samce szczurów szczepu Wistar otrzymywały PB w dawce wynoszącej 98,2 mg/ kg m.c. x dzień-1 jednorazowo, 3-krotnie i 14-krotnie. Wykazano, że PB wywoływał hipermetylację w badanych sekwencjach rejonu promotorowego genu p53. Indukowana PB hipermetylacja genu występowała w przebiegu całego okresu doświadczalnego. Stymulację syntezy DNA, która poprzedzała wzrost masy wątroby oraz indukcję DNMTs, wykazano tylko po 1 i 3 dawkach związku. Kontynuowanie narażenia zwierząt na PB (14 dawek) nie wywoływało zmian w syntezie DNA i aktywności DNMTs w porównaniu do kontroli. Przypuszcza się, że indukowana PB de novo metylacja rejonu promotorowego genu p53, nie była związana z aktywnością DNMTs.

Ograniczanie wyników

Mammalian DNA methyltransferases

Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes

Novel procedure for the detection of 5-methylcytosine

Cloning and characterization of M.LmoA118I, a novel DNA: m4C methyltransferase from the Listeria monocytogenes phage A118, a close homolog of M.NgoMXV

Molecular phylogenetics of DNA 5mC-methyltransferases

Cloning and preliminary characterization of a GATC-specific beta2-class DNA:m6A methyltransferase encoded by transposon Tn1549 from Enterococcus spp.

Site-directed mutagenesis defines the catalytic aspartate in the active site of the atypical DNA: m4C methyltransferase M.NgoMXV

Role of epigenetic DNA alterations in the pathogenesis of systemic lupus erythematosus

Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells

DNA methylation in states of cell physiology and pathology

DNA methyltransferases of Neisseria gonorrhoeae

Cloning of the dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1

Indukowana fenobarbitalem hipermetylacja rejonu promotorowego genu p53 w watrobie szczurow szczepu Wistar