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Okreslenie sekwencji kodujacej region VP1 wirusa pryszczycy typu O

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Paprocka G., Plucienniczak G., Plucienniczak A., Kesy A., Niedbalski W., Fitzner A.

Medycyna Weterynaryjna

1996

tom 52

nr 02

111-113

A Polish strain of foot-and-mouth disease virus type О from the laboratory virus collection was used. The virus was propagated in BHK-21 monolayer cells. Total RNA was extracted by the Chomczyński and Sacchi method. A synthesis of cDNA was performed with AMV-reverse transcriptase. The first and second cDNA strands were used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS (+). The first DNA strand expressed in the phage M13K07 was sequenced according to the Sanger method. The nucleotide sequences were compared to the earlier data published. Genetic similarities to the Brasilian strain of FMDV O1 Campos 58 were found.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Fitzner A.

1 Kesy A.

1 Niedbalski W.

1 Paprocka G.

1 Plucienniczak A.

1 Plucienniczak G.

1 1996

Wyniki wyszukiwania

Okreslenie sekwencji kodujacej region VP1 wirusa pryszczycy typu O