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  1. 4 Cellular and Molecular Biology Letters

  2. 4 Folia Histochemica et Cytobiologica

  3. 4 Folia Histochemica et Cytobiologica. Supplement

  4. 2 Acta Biochimica Polonica

  5. 2 Archivum Immunologiae et Therapiae Experimentalis

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  1. 3 Piasecka M.

  2. 3 Widlak P.

  3. 2 Laszczynska M.

  4. 1 Banas T.

  5. 1 Basta P.

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  1. 2 2019

  2. 1 2015

  3. 3 2013

  4. 1 2012

  5. 1 2009

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1

Expression of IL-3 in WEHI3B cells during apoptosis

100%
Kawiak J., Glowacka E., Hoser G.
Folia Histochemica et Cytobiologica. Supplement
|
1997
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tom 35
|
nr 2
2

Site-specific excision or protection of an alpha a globin gene genomic site in apoptotic transformed chicken erythroblasts

100%
Sjakste N.
Cellular and Molecular Biology Letters
|
2004
|
tom 09
|
nr 3
There is still no clarity on whether the endonuclease incisions in apoptotic cells are induced randomly in the genome or induced in some preferable sites. In order to evaluate the intensity of DNA fragmentation in the chicken alpha-globin domain, AEV-virus transformed chicken erythroblasts (HD3) were incubated in a serum free medium, and their DNA was Southern blotted and hybridised with probes representing different fragments of the domain. Probes corresponding to the upstream areas of the domain mostly hybridised with high molecular weight DNA. Unlike these, the probe corresponding to the 2 Kb BamHI-BamHI fragment, containing the alphaA globin gene (B18), revealed a 5 Kb band on the hybridisation autoradiographs. The probe to the neighbouring upstream fragment did not reveal this band, but it was clearly seen on hybridisations with a downstream 1 Kb BamHI-BamHI fragment. The intensity of the band increased with overall apoptotic DNA degradation, hence its appearance should be coupled to apoptosis. Hybridisation of BamHI-digested DNA with B18 probe revealed a shortening of the 2 Kb band in preparations of DNA from apoptotic cells. The presumable positions of the cuts correspond to the formerly described DNase hypersensitive sites in the domain. Slot-blot and Northern hybridisation of RNA extracted from apoptotic HD3 cells revealed that the excision of the area of the B18 gene is coupled to a decrease in the intensity of alphaA globin gene transcription. Transcription of the non-erythroid NIK gene, transcribed in the upstream part of the domain, did not depend on the level of apoptotic DNA fragmentation.
3

Analysis of in vivo- and in vitro-derived pig expanded blastocysts based on DNA fragmentation

86%
Bryla M., Trzcinska M., Wieczorek J.
Animal Science Papers and Reports
|
2009
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tom 27
|
nr 1
59-68
The aim of the study was to investigate the competence of in vivo- and in vitro-derived pig expanded blastocysts by analysing DNA fragmentation using TUNEL. A total of 533 porcine expanded blastocysts were examined, and results were evaluated using Fisher’s test. Significant differences in the incidence of fragmented nuclei (detected by the TUNEL reaction) and all nuclei (detected by DAPI) were identified between in vivo- and in vitro-derived embryos at the expanded blastocyst stage. The total numbers of nuclei observed in in vivo-derived embryos were significantly different from those in in vitro-cultured embryos (89.1±13.4 and 47.7±25.1, respectively). TUNEL index in In vitro-cultured embryos (28.3%) was significantly higher (P<0.01) than in in vivo-derived blastocysts (4%). These findings indicate that in vivo- and in vitro-derived expanded blastocysts consisting of a small number of cells are characterized by a high incidence of DNA fragmentation. The total number of nuclei in in vivo- and in vitro-derived blastocysts correlated negatively with the number of TUNEL-positive nuclei (r = -0.51; P<0.0001) and the TUNEL index (r = -0.69; P<0.0001), whereas the number of TUNEL-positive nuclei was positively correlated with the TUNEL index (r = 0.95;P<0.0001). Moreover, significant differences were observed between embryos collected from individual experiments.
4

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

86%
Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.
Folia Morphologica
|
2005
|
tom 64
|
nr 4
248-252
Intracerebral haematoma was produced in 25 adult rats by infusion of 100 µl of autologous blood into the striatum. The animals’ brains were removed at 1, 3, 7, 14 and 21 days after production of the haematoma. The TUNEL method was used to detect DNA fragmentation and TUNEL-positive cells were qualified. TUNEL-positive cells were already found on the first day of observation and were present for three weeks after haematoma production. These results provide evidence that programmed cell death is associated with intracerebral haemorrhage.
5

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

86%
Pastuszek E., Kiewisz J., Kulwikowska P. M., Lukaszuk M., Lukaszuk K.
Folia Histochemica et Cytobiologica
|
2015
|
tom 53
|
nr 4
6

The impact of sedentary work on sperm nuclear DNA integrity

86%
Gill K., Jakubik J., Kups M., Rosiak-Gill A., Kurzawa R., Kurpisz M., Fraczek M., Piasecka M.
Folia Histochemica et Cytobiologica
|
2019
|
tom 57
|
nr 1
7

The genotoxicity of amsacrine in normal cells and cancer cells

86%
Blasiak J., Gloc E.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
8

Does the apoptotic fragmentation of DNA starts by excision of chromosomal DNA loop domains?

86%
Ioudinkova E., Bystritsky A., Razin S.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 2
In this paper we are presenting a critical review of works demonstrating that at the early stages of apoptosis eukaryotic DNA is cleaved to large fragments the length of which is several tens of thousands of nucleotide pairs. Evidence is presented that this fragmentation proceeds in a specific fashion and that the character of the fragmentation reflects the general principle of eukaryotic genome organization into structural-functional domains.
9

Autophagy, apoptosis, mitoptosis and necrosis: interdependence between those pathways and effects on cancer

72%
Chaabane W., User S. D., El-Gazzah M., Jaksik R., Sajjadi E., Rzeszowska-Wolny J., Los M. J.
Archivum Immunologiae et Therapiae Experimentalis
|
2013
|
tom 61
|
nr 1
10

Reassessment of the systematic position of Orthocomotis DOGNIN (Lepidoptera: Tortricidae) based on molecular data with description of new species of Euliini

72%
Razowski J., Tarcz S., Wojtusiak J., Pelz V.
Folia Biologica
|
2013
|
tom 61
|
nr 1-2
11

Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

72%
Siedlecka-Kroplewska K., Jozwik A., Kaszubowska L., Kowalczyk A., Boguslawski W.
Folia Histochemica et Cytobiologica
|
2012
|
tom 50
|
nr 4
12

The differences in RCAS1 and DFF45 endometrial expression between late proliferative, early secretory, and mid-secretory cycle phases

72%
Popiela T. J., Wicherek L., Radwan M., Sikora J., Banas T., Basta P., Kulczycka M., Grabiec M., Obrzut B., Kalinka J.
Folia Histochemica et Cytobiologica. Supplement
|
2007
|
tom 45
|
nr 1
157-162
13
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Content available

Involvement of reactive carbonyl species in oxidative stress-induced programmed cell death in tobacco BY-2 cells

72%
Biswas S., Mano J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 2
14

The DFF40-CAD endonuclease and its role in apoptosis

72%
Widlak P.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
The sequential generation of large-scale DNA fragments followed by internucleosomal chromatin fragmentation is a biochemical hallmark of apoptosis. One of the nucleases primarily responsible for genomic DNA fragmentation during apoptosis is called DNA Fragmentation Factor 40 (DFF40) or Caspase-activated DNase (CAD). DFF40/CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. DFF40/CAD is activated by caspase-3 that cuts the nuclease's inhibitor DFF45/ICAD. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and DFF40/CAD is potentially involved in large-scale DNA fragmentation as well. DFF40/CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.
15

DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

72%
Widlak P.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 3
Sequential cleavage of genomic DNA into large-scale DNA fragments of 50-300-kb, followed by formation of mono- and oligonucleosomal DNA fragments, is a biochemical hallmark of programmed, cell death (apoptosis). The endonuclease DFF40/CAD mediates regulated internucleosomal DNA fragmentation and chromatin condensation in cells undergoing apoptosis. DFF40 hypersensitive sites were detected in purified HeLa cell nuclei, and excision of 50-kb DNA fragments preceded formation of oligonucleosomal DNA ladders in nuclei treated with the nuclease. Topoisomerase II, but not topoisomerase I, stimulates DFF40 activity on plasmid DNA substrates. This suggests that interactions of DFF with the nuclear matrix-bound topoisomerase II may be involved in formation of DFF40 hypersensitive sites.
16

Sperm DNA adducts impair fertilization during ICSI but not during IVF

58%
Horak S., Olejek A., Widlak P.
Folia Histochemica et Cytobiologica. Supplement
|
2007
|
tom 45
|
nr 1
99-104
17

Flow cytometry application in the assessment of sperm DNA integrity of men with asthenozoospermia

58%
Piasecka M., Gaczarzewicz D., Laszczynska M., Starczewski A., Brodowska A.
Folia Histochemica et Cytobiologica. Supplement
|
2007
|
tom 45
|
nr 1
127-136
18

Influence of addition of cobalamin to the extender on the post-thaw motility, viability, and DNA integrity of bovine ejaculate

58%
Mizera A., Kuczaj M., Szul A., Jedraszczyk J.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 03
The present study was undertaken to investigate the effect of various concentrations of cobalamin (vitamin B₁₂) in Bioexcell® extender on the spermatozoa quality of Simmental bulls. The semen was collected from 12 bulls by means of an artificial vagina. Vitamin B₁₂ at concentrations of 1.50, 2.50, 3.50, and 5.00 mg/mL was added to bovine Bioexcell® extender. The semen aliquots were cooled and preserved at 4°C. Their quality was evaluated during pre-freezing, and then the cooled semen samples were packaged into 0.25 ml straws. The straws were frozen in the vapor of liquid nitrogen, and stored at -196ºC in a container. The straws were thawed one day later, and the characteristics of spermatozoa were examined. The results showed that the effect of vitamin B₁₂ on the viability, DNA fragmentation and motility of spermatozoa was significant under both pre- and post-freezing conditions (p <0.05), and revealed that supplementation of the extender with B₁₂ improved the post-thaw spermatozoa quality in bulls.
19

Effect of aging on UVC-induced apoptosis of rat splenocytes

58%
Radziszewska E., Piwocka K., Bielak-Zmijewska A., Skierski J., Sikora E.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 2
UVC-induced apoptotic symptoms such as morphological changes, DNA fragmentation, Bcl-2 and Bax protein expression were examined in primary splenocyte cultures from young (3 months) and old (24 months) rats. The activities of AP-1 and CRE transcription factors in UVC-irradiated splenocytes were also assessed. At 24 h after UVC irradiation 40% of cells derived from young rats were found to be apoptotic, which was twice as much as in splenocytes from old rats. Apoptosis in cells from old rats did not give typical symptoms like a DNA ladder and Bcl-2 protein downregulation, in contrast to splenocytes from young rats. No AP-1 transcription factor activity was found in UVC-irradiated splenocytes from old animals and only a trace activity in splenocytes from young animals. This indicates that, UVC-induced apoptosis in rat splenocytes is practically AP-1 independent and that cells from old rats are less sensitive to UVC irradiation than splenocytes from young rats.
20
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Content available

Role of basic fibroblast growth factor in the suppression of apoptotic caspace-3 during chronic gastric ulcer healing

58%
Slomiany B. L., Piotrowski J., Slomiany A.
Journal of Physiology and Pharmacology
|
1998
|
tom 49
|
nr 4
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