Wyniki wyszukiwania

1

Investigation of DNA structures with scanning tunneling microscope

100%

Olinski R., Zastawny T. H., Olejniczak W., Wojczuk S., Chwialkowski M.

Acta Biochimica Polonica

|

1992

|

tom 39

|

nr 1

2

A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

88%

Ciesielska E., Szmigiero L.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 4

A fast two step method of agarose gel electrophoresis for separation of different conformational forms of DNA is described. In the first step the gel is run in the buffer without ethidium bromide and then the gel is stained with that dye. After staining the second step of electrophoresis is performed in the buffer without ethidium bromide. This two step procedure allows one to receive very good resolution between bands corresponding to relaxed, supercoiled, open circular, and linear forms of DNA.

3

The decisive role of the water structure in changes of conformation of nucleic acids

75%

Barciszewski J., Jurczak J., Porowski S., Specht T., Erdmann V. A.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 1

This review summarizes data on the structure and properties of water under normal conditions, at high salt concentration and under high pressure. We correlate the observed conformational transitions in nucleic acids with changes in water structure and activity, and suggest a mechanism of conformational transitions of nucleic acid involving these changes. We conclude that the Z-DNA form is induced only at low water activity caused by high salt concentrations and/or high pressure.

4

Plasmid as a model system for studying DNA damage

63%

Blasiak J., Kowalik J., Walter Z.

Acta Biologica Cracoviensia. Series Zoologia

|

1998

|

tom 40

5

Mutagenesis and ultraviolet inactivation of transforming DNA of Haemophilus influenzae complexed with a Bacillus subtilis protein that alters DNA conformation

51%

Setlow J. K., Setlow B. C., Setlow P.

Acta Biochimica Polonica

|

1996

|

tom 43

|

nr 1

The wild-type Bacillus subtilis spore protein, SspCwt, binds to DNA in vitro and in vivo and changes the conformation of DNA from B to A. Synthesis of the cloned SspCwt gene in Escherichia coli also causes large increases in mutation frequency. Binding of SspCwt to transforming DNA from Haemophilus influenzae made the DNA resistant to ultraviolet (UV) radiation. The mutant protein, SspCala, which does not bind DNA, did not change the UV resistance. The UV sensitivity of the DNA/SspCwt complex was not increased when the recipients of the DNA were defective in excision of pyrimidine dimers. These data indicate that the H. influenzae excision mechanism does not operate on the spore photoproduct formed by UV irradiation of the complex. Selection for the streptomycin- or erythromycin-resistance markers on the transforming DNA evidenced significant mutations at loci closely linked to these, but not at other loci. SspCwt apparently entered the cell attached to the transforming DNA, and caused mutations in adjacent loci. The amount of such mutations decreased when the transforming DNA was UV irradiated, because UV unlinks linked markers.

Ograniczanie wyników

3 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Zoologia

1 Cellular and Molecular Biology Letters

1 Barciszewski J.

1 Blasiak J.

1 Chwialkowski M.

1 Ciesielska E.

1 Erdmann V. A.

1 Jurczak J.

1 Kowalik J.

1 Olejniczak W.

1 Olinski R.

1 Porowski S.

1 Setlow B. C.

1 Setlow J. K.

1 Setlow P.

1 Specht T.

1 Szmigiero L.

1 Walter Z.

1 Wojczuk S.

1 Zastawny T. H.

1 1999

1 1998

2 1996

1 1992

Investigation of DNA structures with scanning tunneling microscope

A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

The decisive role of the water structure in changes of conformation of nucleic acids

Plasmid as a model system for studying DNA damage

Mutagenesis and ultraviolet inactivation of transforming DNA of Haemophilus influenzae complexed with a Bacillus subtilis protein that alters DNA conformation