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Colletotrichum dematium was isolated from caraway for the first time in Poland in 2005. Isolations of this fungus were repeated in 2006 and 2007. The cultures of fungus were obtained from superficially disinfected leaves, root necks, roots, stems and umbels. The isolates were identified on culture media: PDA and malt agar with addition of pieces of caraway stems and on the base of macro and microscopic structures. Studies on the biotic effect between C. dematium and other species of phyllosphere fungi of caraway showed that the majority of the studied species limited the growth and development of C. dematium, but the size of the limiting effect was different. The species from Trichoderma and Gliocladium genera were the most effective against C. dematium, causing degeneration and lysis of hyphae and precluded the formation of the pathogen's acervuli and conidia. C. dematium in dual culture with E. purpurascens, A. radicina, S. sclerotiorum, B. cinerea and R. solani produced an inhibition zone which indicated its capacity for antibiosis.
The aim of present work was to determine pathogenicity of three Colletotrichum dematium isolates to caraway. The effect of post-culture liquids and water suspension of conidia on germination of caraway schizocarps was studied in laboratory conditions. The effect of C. dematium on shooting up and healthiness of the seedlings was carried out in the climatic chamber using the method with infested soil and solidified plaster of culture medium overgrown by the mycelium of the pathogen. The studied isolates of fungus made the germination of parts of tested schizocarps impossible. On the other hand, all isolates caused necrosis of germs and roots of the other germinated schizocarps. The method with Colletotrichum dematium post-culture liquids was recognized as the best to a fast estimation of pathogenicity of fungus to caraway. Based on the positive results of pathogenicity tests the studied isolates of C. dematium were recognized as pathogenic to caraway. Considering this fact and repeated frequency of isolation of the fungus from various organs of this plant (M a c h o w i c z - S t e f a n i a k , 2010), C. dematium was recognized as a potentially pathogenic species to caraway.
In 2004–2006 there were studies conducted on fungi colonizing and harming different parts of plants on plantations of sage (Salvia officinalis L.) grouped in south-eastern Poland. Fungi were isolated from roots, the stem base and leaves. The surface was disinfected by means of a mineral medium. Fungi from genera Fusarium, Rhizoctonia solani, Phoma exigua var. exigua were isolated from roots and the lower parts of stems with the symptoms of necrosis and tissue disintegration. The species Phomopsis sclarea, which had not been observed earlier in Poland, was obtained from the stems with the symptoms of necrosis, peeling off and bark breaking. Altenaria alternata was commonly isolated from the leaves with necrotic symptoms.
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