Qualitative and quantitative contents of free amino acids in the flag leaves and ears of winter tritica- le treated with herbicide Chwastox D 179 SL were determined, using ion-exchange chromatography. Fifteen protein amino acids were identified and determined. The obtained results showed that herbicide treatment did not influence the qualitative composition of free amino acids in the tested winter triticale. The quantitative analysis proved a decrease of the studied compounds in plants treated with Chwastox D 179SL.