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Chlamydophila abortus is one of the most important causative agents of enzootic abortion and other chlamydial infections of sheep and goats. The presence of specifi c serum antibodies to Chlamydophila abortus was studied in sheep and goats breeding in the Slovak Republic by the complement fi xation test. 22,040 sheep and goats were examined during 5 years. Specifi c anti-Ch. abortus antibodies were found in 2,360 out of 20,878 sheep sera examined (11.7%), and in 85 out of 1,162 examined goats (7.7%). The occurrence of antichlamydial antibodies indicates the importance of performing screening examinations in commercial breeding with the aim of reducing the spread of this disease between animals, and also interrupting the spread and transmission from animals to human.
In the presented study, omp-1 gene coding main outer membrane protein of Chlamydophila abortus was cloned into pCI-neo and pcDNA3.1 as delivery vehicles for DNA vaccination. Thirty-six BALB/c mice were randomly assigned to three groups and inoculated intramuscularly with: 1) 100 µg of pCI-neo, 2) 100 µg of pCI-neo::MOMP, and 3) 100 µg of pcDNA3.1::MOMP. All animals were vaccinated three times at 14 d intervals. The results showed that mice given pCI-neo::MOMP developed a higher IgG antibody level, high T lymphocytes proliferations, and high titres of IFN-γ and IL-2, than mice given pcDNA3.1::MOMP, which induced moderate antibody levels, less T lymphocyte proliferations and lower cytokine levels. No significant difference of TNF-α was observed in above groups. Additionally, IgG2a and Ig2b were the predominant isotypes on day 44, suggesting a high level of Th1 stimulation. Mice given the pCI-neo::MOMP also elicited a higher chlamydial clearance and a better protection than mice with pcDNA3.1::MOMP did. Immunisation with pCI-neo::MOMP vaccine may provide novel ways for active immunisation strategy against Chlamydophila abortus.
The aim of performed study was to determine the level of enzootic abortion (EA) in sheep breeding farms in different districts of Lithuania, to determine differences in clinical signs and infection frequency between various age groups, and to evaluate the sensitivity and specificity of complement fixation test for antibodies detection and indirect immunofluorescence for antigen detection in sheep chlamydiosis. The clinical, serological and immunological tests in sheep farms were performed in 2004 and 2005. Comparing different age groups of sheep revealed that the lowest number of infected sheep was registered in animals younger than 18 months (23.1%, antibodies titre 3.191 log2, P<0.05) and highest in animals aged 18 to 24 months (53.8%, antibodies titre 4.224 log2, P<0.001). In sheep aged more than 3 years, titre of antibodies was significantly reduced. The majority of infected sheep which aborted (86.4%) was registered in 18-24 month age group. Furthermore, in sheep which aborted the infection level was 2.5-fold higher as compared to sheep which didn’t abort. Analysis of smears from patological material by indirect FAT revealed that 54.5% of animals were positive to Chlamydophila abortus infection. The highest prevalence of chlamydia (66.7%) was registered in placentas of sheep which aborted.
An epidemiological study was carried out to determine the herd prevalence of Chlamydophila abortus and Coxiella burnetii antibodies in goats covered by a milk recording program in Poland. The survey took place in 2007 and 48 herds located in different parts of the country were involved. A representative sample from each herd was taken by a simple random sampling allowing to detect seropositivity of a herd on a 95% level of confidence. In total 918 goats were tested for specific antibodies against both germs with the use of enzyme-linked immunosorbent assays. In addition, history of reproductive failures was recorded in these herds. The survey revealed that the herd prevalence of C. abortus was 4.2% (2 herds) while no C. burnetii antibodies were found. Abortions were reported to be a problem in 80% of herds while repeating estrus was encountered in 46% of herds. Reproductive failure concerned two seropositive herds as well. Since the germ is present in the population, it has to be taken into consideration in diagnostic process. Nevertheless, the results of the present study indicate that C. abortus infection occurs infrequently in Polish goats. As no antibodies against C. burnetii were detected in the screened sample the risk of goat-to-human transmission of both bacteria in Poland seems to be very low.
The aim of the study was to determine the seroprevalance of Chlamydophila abortus in aborting ewes and cattle in the Kars province in the north-eastern part of Turkey. Thirty sheep flocks and 26 herds of dairy cattle out of 4 districts in the province were examined by ELISA. For this purpose, abortion cases of 236 sheep and 192 cows of different breeds and age were investigated serologically during lambing and calving season. Subsequently, the rate of abortion in ewes and cows were 10.25% (236/2302) and 28.23% (192/680), respectively. Antibodies against C. abortus were found in 46.66% (14/30) of sheep flocks and 26.92% (7/26) cattle herds examined. In the study, 13.98% (33/236) of aborted sheep and 8.33% (16/192) of cattle were positive for antibodies specific to C. abortus. Seroprevalence of C. abortus in sheep and cattle ranged from 5.40 to 18.29% and 4.76 to 12.67%, respectively. In conclusion, C. abortus causes abortion in both sheep and dairy cattle in the north-eastern part of Turkey. It is recommended that seropositive animals should be eliminated from flocks and herds. An appropriate vaccine against C. abortus should also be applied for ewes and cows to reduce the incidences of abortion.
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Enzootyczne ronienie owiec

67%
The following species of the family Chlamydiaceae are the most important in causing asymptomatic or symptomatic infections in swine: Chlamydophila abortus, Chlamydophila pecorum, Chlamydophila psittaci and Chlamydia suis. Mostly they cause asymptomatic infections or to an unknown percentage they participate in the etiology of multifactorial syndromes, usually with other species of facultatively pathogenic bacteria or viruses. Chlamydiaceae in pigs are not exclusive etiological agents of strictly defined diseases, as for example Chlamydia trachomatis in causing trachoma in humans, but are associated with different pathologies such as: conjunctivitis, pneumonia, pericarditis, polyarthritis, enteritis, return to estrus, abortion, mummification of fetuses or piglets before parturition, or abortion, delivery of weak piglets and increased perinatal or neonatal mortality. The mentioned chlamydial species also contribute to inferior semen quality. However, in comparison with infections or diseases of pigs caused by other microorganisms, Chlamydiaceae are at present considered as rather less important pathogens. Whether this evaluation is a proper one has to be considered in the future, since diagnostic laboratories rarely routinely investigate specimens from swine for Chlamydiaceae. In the review diagnostic tests for the identification of Chlamydiaceae were mentioned as well, with an indication of their diagnostic value. In the introduction, remarks concerning the taxonomy of Chlamydiaceae were presented.
Medycyna Weterynaryjna
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2010
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tom 66
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nr 09
s.584-587,bibliogr.
Properties of chlamydia organisms were presented, including their unique growth cycle. Changes in the taxonomy were characterised. The changes are due to the use of molecular biology and the analysis of the genes 16S and 23S rRND. As a result, the order Chlamydiales contains now at least four families. Among them is the family Chlamydiaceae with two genera: Chlamydia and Chlamydophila. The genus Chlamydia includes C. trachomatis, C. muridarium and C. suis, whereas the genus Chlamydophila comprises Cp. pneumoniae, Cp. pecorum, Cp. psittaci, Cp. abortus, Cp. caviae, and Cp. felis. Cp. psittaci causes avian chlamydiosis and Cp. abortus is responsible for ovine chlamydiosis. Moreover, these species and other above-mentioned species participate in causing pathological syndromes of polyetiological character. Also swine are susceptible to these syndromes. Therefore, as a rule, this animal species suffers only from infections by chlamydia in which also other microorganisms participate and not from chlamydiosis caused exclusively by one species of chlamydia, as birds or sheep. Clinical sings of pathological syndromes caused jointly by chlamydia and other microorganisms in swine are: pneumonia, diarrhea, conjunctivitis, pericarditis, abortion and other reproductive disorders, also in boars. Antibiotics effective in chlamydial infections of swine were mentioned. Vaccines are not available. Diagnostic laboratory methods were characterized. The methods have been improved by the introduction of PCR, which makes it possible to identify chlamydia species directly from clinical specimens.
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