The identification of twenty four yeast isolates from cheese performed with API ID 32C simplified assimilation test and restriction fragments length polymorphism (PCR-RFLP) of ITS1-5.8S rDNA-ITS2 region was compared. The accordance of the results of both methods for eleven strains identified as Candida famata, five strains as C. lipolytica and four strains as C. sphaerica was observed. However, the other four yeast isolates classified with API as C. sphaerica revealed amplification products as well as restriction patterns characteristic for C. famata species.
The effect of various concentrations of salt (0%, 0.7%, 1.5% and 3.6% of sodium chloride) on enzymatic activity of C. famata, C. lipolytica, C. colliculosa, C. holmi and C. albicans isolated from the Szczecin Lagoon was tested. The activity revealed low enzymatic activity of Candida isolated deceasing with the rise in concentration of NaCl in the medium. C. colliculosa expressed the lowest hydrolytic activity regardless of the salt concentration in the medium. The highest enzymatic activity at 0% of NaCl was demonstrated by C. lipolytica (15.16 nmol) and C. albicans (14.79 nmol).