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Modulation of ERK1-2 activity is crucial for sphingosine-induced death of glioma C6 cells

100%

Krzeminski P.

Acta Biochimica Polonica

2005

tom 52

nr 4

In this study the contribution of the ERK1/2 pathway to sphingosine-induced death and morphological changes of the actin cytoskeleton in glioma C6 cells was investigated. Surprisingly, the level of ERK1/2 phosphorylation does not change after incubation of cells with sphingosine. Despite this, sphingosine induces rounding and detachment of cells without formation of apoptotic bodies. To shed light on this process, a specific inhibitor of ERK1/2 phosphorylation, U0126, was used. Cells incubated simultaneously with sphingosine and U0126 not only detached, but also exhibited formation of apoptotic-like blebs. These data suggest that during sphingosine-induced glioma C6 cell death apoptotic blebbing is dependent on ERK1/2 signalling and occurs only when ERK1/2 activity is decreased or abolished.

Cross-talk between the ATP and ADP nucleotide receptor signalling pathways in glioma C6 cells

72%

Czajkowski R., Baranska J.

Acta Biochimica Polonica

2002

tom 49

nr 4

In this review we summarize the present status of our knowledge on the enzymes involved in the extracellular metabolism of nucleotides and the receptors involved in nucleotide signalling. We focus on the mechanism of the ATP and ADP signalling pathways in glioma C6, representative of the type of nonexcitable cells. In these cells, ATP acts on the P2Y2 receptor coupled to phospholipase C, whereas ADP on two distinct P2Y receptors: P2Y1 and P2Y12. The former is linked to phospholipase C and the latter is negatively coupled to adenylyl cyclase. The possible cross-talk between the ATP-, ADP- and adenosine-induced pathways, leading to simultaneous regulation of inositol 1,4,5-trisphosphate and cAMP mediated signalling, is discussed.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Baranska J.

1 Czajkowski R.

1 Krzeminski P.

1 2005

1 2002

Wyniki wyszukiwania

Modulation of ERK1-2 activity is crucial for sphingosine-induced death of glioma C6 cells

Cross-talk between the ATP and ADP nucleotide receptor signalling pathways in glioma C6 cells