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Field examination of the boviseptivac vaccine and measurement of the IgG anti-Pasteurella multocida level by the ELISA

100%

Borkowska-Opacka B., Kedrak A., Truszczynski M., Rutkowska-Jurga I.

Bulletin of the Veterinary Institute in Puławy

1998

tom 42

nr 1

The goal of the experiment was to evaluate the Boviseptivac vaccine against cattle pasteurellosis manufactured from local isolates of P. multocida serotype B:2 with the addition of an oil adjuvant (Emulsigen). Field examinations were performed on 645 cows in the Ostroleka province. The vaccine effectively protected against the disease; however, 6 (93%) of examined cows revealed shock signs which were lessened with anti-shock preparations. The immunogenicity of the vaccine was determined by the agar gel diffusion precipitin test and ELISA. Examined blood was sampled from 10 cows before vaccination and 2, 4 and 8 months thereafter. The vaccine stimulated the production of specific precipitins to serotype 2 and increased levels of the IgG anti-P. multocida.

Preliminary investigation into the immunisation of cows against pasteurellosis using boviseptivac vaccine

75%

Borkowska-Opacka B., Kedrak A., Truszczynski M.

Bulletin of the Veterinary Institute in Puławy

1997

tom 41

nr 2

Investigations aimed to evaluate the effectiveness of Boviseptivac, vaccine against bovine pasteurellosis prepared from the Polish isolates of serotype B:2 with an oil adjuvant (Emulsigen). The vaccine protective value obtained on white mice was 4.25 according to the method of Ose and Muenster. Field trials carried out on 120 cows in the Ostroleka province revealed advantageous properties of the vaccine, such as a high effectiveness and the lack of lesions at the site of injection. Moreover, no health disturbances in cows were observed. The vaccine immunogenicity was evaluated serologically. Blood collected from 10 cows was examined before vaccinaton and 1 month postinjection. Immunisation stimulated the production of precipitins specific for serotype 2 demonstrated by the precipitin diffusion reaction on agar gel. The ELISA enabled us to detect an increased lgG level after immunisation.

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

2 Borkowska-Opacka B.

2 Kedrak A.

2 Truszczynski M.

1 Rutkowska-Jurga I.

1 1998

1 1997

Wyniki wyszukiwania

Field examination of the boviseptivac vaccine and measurement of the IgG anti-Pasteurella multocida level by the ELISA

Preliminary investigation into the immunisation of cows against pasteurellosis using boviseptivac vaccine