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The objective of undertaken studies was evaluation in vitro conditions efficiency of eight fungicides from different chemical groups and three preparations of natural origin i.e. Biosept Active, Beta-chikol and Bioczos towards isolate M 365 Boeremia strasseri, obtained from peppermint rhizomes showing symptoms of black rot. Test were performed by poisoning the culture media. The percentage of inhibition of the growth of four- and eight-day-old colonies on the medium with preparations in the control colonies was a measure of activity of the preparations. Moreover, the microscopic observations of morphological structures of fungus were conducted. Within chemical compounds trifloxystrobin, tiophanate methyl and mancozeb pointed to be the most effective. The effectiveness of Beta-chikol and Biosept Active in limiting B. strasseri colony growth was higher than the efficiency of Bioczos.
The biotechnical preparations: Biosept Active (based on a grapefruit extract) and BioZell (based on thyme oil) as well as Hypericum perforatum extract, streptomycin solution and fungicide Champion 50WP (active ingredient substance – e.i. 50% copper hydroxide) were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on three media: Nutrient Agar (NA Difco), Pseudomonas Agar F (Merck) – analogue of King B and 523. In the experiments, the agar plate method was applied. There were no statistically significant differences in the effect of streptomycin and Champion 50WP on the growth inhibition of three bacteria strains for medium 523 and Nutrient Agar and of P. syringae pv. syringae and X. arboricola pv. corylina for medium King B. It was determined that the antibacterial activity of Biosept Active and BioZell biopreparations and H. perforatum extract against Agrobacterium tumefaciens (strain C58), Pseudomonas syringae pv. syringae (strain 760) and Xanthomonas arboricola pv. corylina (strain RIPF-x13) were dependent on the strain of pathogen as well as the growth medium used. According to the research results obtained, the Biosept Active preparation and H. perforatum extract demonstrated high bacteriostatic activity against three bacterial strains grown on the Nutrient Agar medium.
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