In present study the essential oils composition and antioxidant activities of the essential oils as well as methanolic extracts of leaves, fruits and roots of Biebersteinia multifida were evaluated. The GC-MS analysis of the essential oils resulted in the identification of 36 compounds. Thymol (16.5–38.4%), β-caryophyllene (9.8–15.5%), 1,8-cineol (5.8–18.4%), α-pinene (0.9–14.3%) and β-pinene (2.3–12.4%) were the main components. The samples were subjected to screening for their possible antioxidant activity by using DPPH and β-carotene-linoleic acid assay. In the first case, the radical scavenging activity of the essential oil and methanolic extract of fruits were superior to all other essential oils and extracts (IC50=16.7). In the case of the linoleic acid system, oxidation of the linoleic acid was effectively inhibited by essential oils and extracts of different parts. The fruit extracts showed 95.4±2.15% inhibition, which is comparible to synthetic antioxidant BHT, curcumine and ascorbic acid.