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1

The release of sulfur compounds during degradation of feather keratin by two Bacillus strains

100%
Laba W., Choinska A., Rodziewicz A.
Acta Scientiarum Polonorum. Biotechnologia
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2013
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tom 12
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nr 2
Keratinolytic bacteria Bacillus polymyxa B20 and B. cereus B5esz during cultures in medium with chicken feathers as a sole nutrient source, accumulated various amounts of sulfur compounds at different oxidation level, including thiols, thiosulfate, sulfite and sulfate. The main difference observed between the two tested strains was higher release of sulfate by the former and elevated concentration of thiols by the latter. Additionally, the activity of glutathione reductase, that could potentially play a role in keratinolysis was confirmed, mainly in the cell homogenate fraction, rather than extracellular. Keratinases in crude culture fluids exhibited activity towards soluble keratin preparation, as well as native feather keratin. Application of 2-mercaptoethanol and sulfite, agents that potentially could take part in keratin sulfitolysis, led to a conclusion that they could play a role in keratin degradation, other than activation of extracellular enzymes.
2

Selective isolation of Bacillus thuringiensis from soil by use of L-serine as minimal medium supplement

100%
Andrzejczak S., Lonc E.
Polish Journal of Microbiology
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2008
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tom 57
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nr 4
The influence of L-serine on the growth of different strains of the genus Bacillus was investigated. It has been observed that the addition of L-serine to minimal synthetic media results in an inhibition in the growth of certain strains of Bacillus spp. but not B. thuringiensis. Then L-serine-resistance phenomenon was used in isolation of B. thuringiensis strains from soil. An isolation method with media supplied with L-serine was compared to the previously applied procedure (isolation on nutrient agar). L-serine-selective medium appeared to be more effective in isolation of Bt strains.
3
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Effect of physical and chemical factors in production of alkaline protease enzyme by Bacillus strains

86%
Tebyanian H., Mirhosseiny S. H., Bakhtiari A., Karami A., Dadseresht S., Otroshi B.
International Letters of Natural Sciences
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2018
|
tom 71
Proteases is family of enzymes and it has crucial role due to their physiological roles and very valuable commercial applications. Alkaline protease are produced by Bacillus species are particular importance because of their thermal stability and stability at different pH values. This study aimed to investigate the effect of physical and chemical factors in production of alkaline protease enzyme fermentation by members of the genus Bacillus. In this study, alkaline protease enzyme production were evaluated in submerged fermentation by Bacillus strains which were isolated from alkaline soils of Guilan province. Factors incubation were optimized such as time, pH, amount of inoculation and ammonium sulfate in alkaline protease enzyme production whit using response surface methodology (RSM) in culture. The maximum enzymatic activity was observed in incubation time of 36 hours, pH=9, inoculation amount of 15% (V) and ammonium sulfate 1.5% (W/V). Factors had significant effect on the production of alkaline protease enzyme such as pH and ammonium sulfate.
4

Exopolysaccharide production by Bacillus strains colonizing packaging foils

58%
Szumigaj J., Zakowska Z., Klimek L.
Polish Journal of Microbiology
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2008
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tom 57
|
nr 4
The influence of the chemical composition of medium, availability of glucose and pH on the production of exopolysaccharides (EPS) by different Bacillus strains were investigated. Bacillus strains were isolated from the surface of polyethylene foils modified with mineral compounds after their biodégradation in compost soil. Moreover, the effect of EPS production on bacterial adhesion onto the surface of the materials was examined. The enhanced synthesis of exopolysaccharides in nutrient-starved conditions was revealed. The most effective synthesis of polymers was observed during the logarithmic phase of culture growth. The increased amount of EPS facilitated bacterial adhesion to material surfaces. It was determined that the biofilm on the material surface positively affects its biodégradation. Based on the results, we conclude that the biodégradation of polymers may be accelerated in low-nutrient environment.
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