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Motifs with potential physiological activity in food proteins - BIOPEP database

100%

Iwaniak A., Dziuba B.

Acta Scientiarum Polonorum. Technologia Alimentaria

2009

tom 08

nr 3

59-85

Proteins are the multifunctional food components affecting the living organ-isms. One of the proteins function is the impact on the body due to the presence of motifs that show specific physiological and biological activities. Due to the worldwide growth of demand for the food containing bioactive components, increasing attention has been paid recently to the use of bioactive peptides as physiologically active food ingredients. They are important elements of the prevention and treatment of various lifestyle diseases. In addition to its primary function and according to current knowledge, each protein may be a reserve source of peptides controlling the life processes of organisms. For this reason, in this work, application of a new, additional criterion for evaluating proteins as a potential source of biologically active peptides, contributes to a more comprehensive and objective definition of their biological value. A complementary part of such research is the strategy for evaluation of the food proteins as precursors of biologically active peptides which involves the database of proteins and bioactive peptides - BIOPEP (available on-line at: http://www.uwm.edu.pl/biochemia). The database contains information on 2123 peptides representing 48 types of bioactivities, their EC50 values and source of origin. Proteins (706 sequences) are considered as bioactive peptide precursors based on newly introduced criteria: the profile of potential biological activity, the frequency of bioactive fragments occurrence and potential biological protein activity. This original and unprecedented so far approach, started to be successfully and more widely applied by other authors. BIOPEP can be interfaced with global databases such as e.g. TrEMBL, SWISS-PROT, EROP and PepBank. Recently the BIOPEP database was enlarged with the data about allergenic proteins, including information about structure of their epitopes and molecular markers

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Bioinformatics-aided characteristics of the structural motifs of selected potentially celiac-toxic proteins of cereals and leguminous plants

67%

Dziuba M., Dziuba J., Iwaniak A.

Polish Journal of Food and Nutrition Sciences

2007

tom 57

nr 4

405-414

A structural analysis was conducted of peptides responsible for inducing celiac disease, as well as of the structural fragments they are located in, i.e. the so-called “extended structural motifs”. These motifs originated from wheat A-gliadin and constituted a standard for the analysis of the other proteins. Experiments were carried out based on in silico methods. Analyses covered a total of 403 sequences of selected cereal and seed proteins. Of all the analysed sequences, 155 were found to contain tetrapeptides (potentially known as toxic for celiac disease), namely: QQQP, QQPY, PSQQ, QPYP, including 29 proteins in which the tetrapeptides were constituents of a structural motif with a longer sequence. These were proteins of wheat, barley and oat. All the extended motifs occurred in a hydrophilic surrounding, attaining the structure of beta-turn and random coil. Computer-aided design of the proteolysis process of selected proteins was carried out as well in the aspect of celiac-toxic peptide release. Active fragments were released from twenty-eight proteins by thermolysin, K proteinase and prolyl oligopeptidase.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Technologia Alimentaria

1 Polish Journal of Food and Nutrition Sciences

2 Iwaniak A.

1 Dziuba B.

1 Dziuba J.

1 Dziuba M.

1 2009

1 2007

Wyniki wyszukiwania

Motifs with potential physiological activity in food proteins - BIOPEP database

Bioinformatics-aided characteristics of the structural motifs of selected potentially celiac-toxic proteins of cereals and leguminous plants