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Background. The aim of this study was isolation and examination of Aspiculuris tetraptera somatic proteins and somatic antigens role in eliciting of immune response in laboratory mice. Material and methods. In our investigation 40 laboratory mice (BALB/c strain) were used. To extract the somatic proteins Tris-HCl buffer with NaCl and Triton X-100 was used. The analysis of somatic antigens was undertaken by Western blotting. Results. The study showed the presence of 14 protein bands ranging from ~ 82 to 28 kDa. Glicoproteins detection revealed 13 bands in range between ~ 70 to 30 kDa. There was no reaction observed with immunoglobulins IgA. Comparision of these results with earlier studies concerning S. obvelata somatic antigens show that there are proteins and glicoproteins with the same molecular weights for both species. It is also observed that S. obvelata somatic extract is more diversed and have higher antigenicity than A. tetraptera. Hence, we may suppose this fact could favour easier colonization of the host by A. tetraptera.
Problems dealing with common parasites - oxyurids, which affect laboratory mice colonies are discussed and reviewed. Their life histories, pathogenicity and immunity are examined. The attention is paid to the influence of age, sex, strain and status of the host in the infection. It is agreed that helminth infections are usually more severe in male than in female vertebrate hosts.
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Helminth parasites of laboratory mice and rats

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Rodents, as mice and rats are the most common laboratory animals used in research and testing. They are seldom investigated for autochthonous ecto- and endoparasites prior their utilization in the experiments. Helminth parasites can alter the interpretation of final results. Pinworms commonly infecting laboratory rodents include mainly the mice pinworms Syphacia obvelata and Aspiculuris tetraptera, and in rats Syphacia muris. The fact that many laboratory rodent colonies were found to be parasite contaminated suggests a need for eradication and improvment of the quality of laboratory rodents. This review reports the data on the presence of helminth parasites in laboratory rodents colonies, and suggests to pay special attention on controlling the sanitary conditions of animal houses.
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